Purpose: The purpose of this pilot study was to assess the acute effects of consuming pre-workout supplements on indices of muscular strength, endurance and mood states.

Methods: In a double- blind, placebo-controlled, randomized crossover design, fourteen moderate to highly-trained recreational athletes (7 female, 7 male) participated in this investigation. Subjects came to the lab twice between testing sessions. They consumed either pre supplement (mixed with 8 to 12 ounces of water) or placebo 30-minutes prior to testing. The pre-workout supplement combination (Athelite Nutrition Inc.) contained 15.62 grams per serving, 25 kcals, that consisted of a proprietary blend including caffeine (as green coffee bean extract), L-theanine, black pepper extract, micronized creatine monohydrate, CarnoSyn® beta-alanine, Huperzine A, N-Acetyl L-carnitine, Nitrosigine®), or placebo. The placebo was a similar tasting drink with an equal amount of caffeine. Their body composition was assessed via the DEXA (Hologic Model Horizon W). Participants’ mood was also assessed via a profile mood states questionnaire (POMS) 30 minutes after product or placebo was consumed. After taking the profile mood states questionnaire, subjects had their exercise performance assessed via the 1-repition maximum bench press followed by bench press repetitions to failure at 60% of 1-repetition maximum with 30 seconds rest between sets (3 total sets).

Results: There were significant differences (p

Conclusion: The results demonstrated that the acute consumption of pre-workout supplements can enhance muscular endurance. Caffeine alone cannot explain effect on muscular endurance since the placebo also contained caffeine. However, the supplements had no effect on strength or mood states.

Author Bio(s)

Anya Ellerbroek, B.A. Sports Exercise Science, CISSN, is the research assistant at the International Society of Sports Nutrition (ISSN) with Dr. Jose Antonio.

Dr. Jose Antonio PhD, FISSN, CSCS, is the president of the International Society of Sports Nutrition (ISSN), and faculty member of the Sports Exercise Science Bachelors- Human Health Science Department, at Nova Southeastern University in Ft. Lauderdale Fl.


We would like to thank Athelite Nutrition Inc. for providing the pre workout supplement. We would like to thank Jose Antonio for editing, proof reading, and guidance throughout the study. We would also like to thank all research assistants for their work during the study. The study was funded by Athletite Nutrition Inc. The authors do not have any competing interests.





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