Photodynamic Inactivation (PDI) of Microorganisms with Nitrogen Substituted Meso-Pyridyl Porphyrin Structures
Abstract
Photodynamic Inactivation (PDI) is an antimicrobial approach targeting pathogens independent of antibiotic resistance. The growing international concern for the rise in drug resistant microorganisms has driven for research in approaches that will keep these microbes from developing any immunological adaptations. Photosensitizers are molecules activated by light that produce reactive oxygen species (ROS) lysing and causing cell death in structures of microbes and in diseased mammalian cells. The photosensitizers applied in this experiment are meso-Tetra(N-methyl-_-pyridyl)porphine tetrachloride structures. Three variations of the nitrogen placement were used to understand the interactions between such and the bacterial cell wall of Escherichia coli. These compounds were selected for their reactivity, allowing for multiple interactions to be observed between each porphyrin and the gram-negative E. coli. Minimum inhibitory concentration (MIC) tests were conducted to determine at what concentration will each porphyrin work most efficiently in eliminating microorganisms. In determining it’s efficiency, the primary objective of this experiment is to advance photodynamic inactivation techniques to be applied to microorganisms eliminating any form of resistance.
Faculty Sponsors
Dr. Maria Ballester, Dr. Robert Smith
Project Type
Event
Location
Alvin Sherman Library
Start Date
4-5-2023 12:00 PM
End Date
4-6-2023 4:00 PM
Photodynamic Inactivation (PDI) of Microorganisms with Nitrogen Substituted Meso-Pyridyl Porphyrin Structures
Alvin Sherman Library
Photodynamic Inactivation (PDI) is an antimicrobial approach targeting pathogens independent of antibiotic resistance. The growing international concern for the rise in drug resistant microorganisms has driven for research in approaches that will keep these microbes from developing any immunological adaptations. Photosensitizers are molecules activated by light that produce reactive oxygen species (ROS) lysing and causing cell death in structures of microbes and in diseased mammalian cells. The photosensitizers applied in this experiment are meso-Tetra(N-methyl-_-pyridyl)porphine tetrachloride structures. Three variations of the nitrogen placement were used to understand the interactions between such and the bacterial cell wall of Escherichia coli. These compounds were selected for their reactivity, allowing for multiple interactions to be observed between each porphyrin and the gram-negative E. coli. Minimum inhibitory concentration (MIC) tests were conducted to determine at what concentration will each porphyrin work most efficiently in eliminating microorganisms. In determining it’s efficiency, the primary objective of this experiment is to advance photodynamic inactivation techniques to be applied to microorganisms eliminating any form of resistance.
