Pilot Study of A375 Melanoma Xenograft in Nu/Nu Mice
Abstract
Pilot studies play an important role prior to conducting a full-scale research study as they determine crucial components of the study, such as sample size and whether or not randomized control trials (RCTs) will be feasible. In this pilot study, 5 male and 5 female Nu/Nu mice that were approximately 25 grams and 7 weeks old were injected with 2 million A375 cells in 0.2 mL of the right flanks of the mice. Once the tumors became palpable, their volumes were measured three times per week using a caliper. Upon completion of the study, average tumor volumes and standard deviations were tabulated and used to estimate sample size for future xenograft studies. As a result, the IACUC protocol was updated to use 14 mice per group based on the pilot study. Based on our findings, plans were made to start studies on the drug-resistant A375 melanoma xenograft efficacy model of the compounds 2155-14 and 2155-18.
Faculty Sponsors
Dr. Dmitriy Minond
Project Type
Event
Location
Alvin Sherman Library
Start Date
4-5-2023 12:00 PM
End Date
4-6-2023 4:00 PM
Pilot Study of A375 Melanoma Xenograft in Nu/Nu Mice
Alvin Sherman Library
Pilot studies play an important role prior to conducting a full-scale research study as they determine crucial components of the study, such as sample size and whether or not randomized control trials (RCTs) will be feasible. In this pilot study, 5 male and 5 female Nu/Nu mice that were approximately 25 grams and 7 weeks old were injected with 2 million A375 cells in 0.2 mL of the right flanks of the mice. Once the tumors became palpable, their volumes were measured three times per week using a caliper. Upon completion of the study, average tumor volumes and standard deviations were tabulated and used to estimate sample size for future xenograft studies. As a result, the IACUC protocol was updated to use 14 mice per group based on the pilot study. Based on our findings, plans were made to start studies on the drug-resistant A375 melanoma xenograft efficacy model of the compounds 2155-14 and 2155-18.
