Molecular Analysis of the Human Serum Amyloid a (SAA) Gene Family

Authors

• G. H. Sack Jr., Johns Hopkins University
• C. C. Talbot Jr., John F. Kennedy Institute
• Hector Seuanez, National Cancer Institute at Frederick
• Stephen J. O'Brien, National Cancer Institute at FrederickFollow

ORCID

0000-0001-7353-8301

ResearcherID

N-1726-2015

Document Type

Article

Publication Title

Scandinavian Journal of Immunology

ISSN

0300-9475

Publication Date

1-1989

Abstract

We have assigned the human serum amyloid A (SAA) gene family to a 90 kb region on the short arm of human chromosome 11 (11p) by hybridization of defined genomic fragments of human SAA genes to DNA from rodent-human somatic cell hybrids and to large DNA fragments separated by transverse alternating field gel electrophoresis. We have also characterized SAA probe hybridization patterns in human DNA cleaved with restriction endonucleases Hind III, Pst I, Bgl II, Taq I, and Xba I and found invariant patterns except for a two-allele restriction fragment length polymorphism (RFLP) with Hind III. These studies show that the SAA gene family comprises at least three members in the haploid human genome and will be useful in identifying variant patterns and establishing linkage between members of the SAA gene family and other markers on chromosome 11.

Volume

29

Issue

1

First Page

113

Last Page

119

Additional Comments

USPHS grant #: AM-31745-03

NSUWorks Citation

Sack, G. H. Jr.; C. C. Talbot Jr.; Hector Seuanez; and Stephen J. O'Brien. 1989. "Molecular Analysis of the Human Serum Amyloid a (SAA) Gene Family." Scandinavian Journal of Immunology 29, (1): 113-119. https://nsuworks.nova.edu/cnso_bio_facarticles/344