Biology Faculty Articles
Title
Human Endothelial Cell Growth Factor: Cloning, Nucleotide Sequence, and Chromosome Localization
Document Type
Article
Publication Date
8-1-1986
Publication Title
Science
ISSN
0036-8075
Volume
233
Issue/No.
4763
First Page
541
Last Page
545
Abstract
Several of the endothelial cell polypeptide mitogens that have been described probably play a role in blood vessel homeostasis. Two overlapping complementary DNA clones encoding human endothelial cell growth factor (ECGF) were isolated from a human brain stem complementary DNA library. Southern blot analysis suggested that there is a single copy of the ECGF gene and that it maps to human chromosome 5 at bands 5q31.3 to 33.2. A 4.8-kilobase messenger RNA was present in human brain stem messenger RNA. The complete amino acid sequence of human ECGF was deduced from the nucleic acid sequence of these clones; it encompasses all the well-characterized acidic endothelial cell polypeptide mitogens described by several laboratories. The ECGF-encoding open reading frame is flanked by translation stop codons and provides no signal peptide or internal hydrophobic domain for the secretion of ECGF. This property is shared by human interleukin-1, which is approximately 30 percent homologous to ECGF.
NSUWorks Citation
Jaye, Michael; Richard Howk; Wilson Burgess; George A. Ricca; Ing-Ming Chiu; Mark W. Ravera; Stephen J. O'Brien; William Modi; Thomas Maciag; and William N. Drohan. 1986. "Human Endothelial Cell Growth Factor: Cloning, Nucleotide Sequence, and Chromosome Localization." Science 233, (4763): 541-545. https://nsuworks.nova.edu/cnso_bio_facarticles/157
ORCID ID
0000-0001-7353-8301
ResearcherID
N-1726-2015
Comments
©1986 American Association for the Advancement of Science