Biology Faculty Articles
Document Type
Article
Publication Date
11-2009
Publication Title
Plant Physiology
ISSN
1532-2548
Volume
151
Issue/No.
3
First Page
1167
Last Page
1174
Abstract
Recent studies have documented that the soybean (Glycine max) genome has undergone two rounds of large-scale genome and/or segmental duplication. To shed light on the timing and nature of these duplication events, we characterized and analyzed two subfamilies of high-copy centromeric satellite repeats, CentGm-1 and CentGm-2, using a combination of computational and molecular cytogenetic approaches. These two subfamilies of satellite repeats mark distinct subsets of soybean centromeres and, in at least one case, a pair of homologs, suggesting their origins from an allopolyploid event. The satellite monomers of each subfamily are arranged in large tandem arrays, and intermingled monomers of the two subfamilies were not detected by fluorescence in situ hybridization on extended DNA fibers nor at the sequence level. This indicates that there has been little recombination and homogenization of satellite DNA between these two sets of centromeres. These satellite repeats are also present in Glycine soja, the proposed wild progenitor of soybean, but could not be detected in any other relatives of soybean examined in this study, suggesting the rapid divergence of the centromeric satellite DNA within the Glycine genus. Together, these observations provide direct evidence, at molecular and chromosomal levels, in support of the hypothesis that the soybean genome has experienced a recent allopolyploidization event.
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
NSUWorks Citation
Gill, Navdeep; Seth Findley; Jason G. Walling; Christian Hans; Jianxin Ma; Jeff Doyle; Gary Stacey; and Scott A. Jackson. 2009. "Molecular and Chromosomal Evidence for Allopolyploidy in Soybean." Plant Physiology 151, (3): 1167-1174. doi:https://doi.org/10.1104/pp.109.137935.
ORCID ID
http://orcid.org/0000-0003-3746-1866
DOI
https://doi.org/10.1104/pp.109.137935
Comments
© 2009 American Society of Plant Biologists © The Author(s) 2009. Published by Oxford University Press on behalf of American Society of Plant Biologists. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.