Adoptive Transfer of B10 Cells Promotes Resolution of Periodontal Inflammation

Adoptive Transfer of B10 Cells Promotes Resolution of Periodontal Inflammation

Alvin Sherman Library

Regulatory B cells (Bregs) play a crucial role in reducing the inflammation of periodontitis. It has been shown that specialized pro-resolving mediator (SPM) synthesis and production mediate the resolution of periodontitis inflammation and alleviate bone resorption. Bregs has been reported alleviate the inflammation in periodontitis, but little is known about their function in SPM production. The present study aims to investigate whether IL-10 expressing regulatory B cells (B10) can inhibit periodontitis progression by promoting the production of SPMs. Experimental periodontitis models were established in mice by ligation combined with periodontal pathogen P. gingivalis bacteria inoculation for 14 days, and B10 cells were adoptively transferred on the 3rd day after ligation. Mass spectroscopy was used to detect the production of SPMs in periodontal gingival tissues, Hematoxylin and Eosin (HE) staining was used to evaluate the inflammatory cell infiltration in periodontitis tissue. Tartrate-resistant acid phosphatase (TRAP) staining was used to detect the number of osteoclasts along the alveolar bone surface. Mass spectroscopy showed increased RvD2 expression in the B10 transfer group compared to the control. HE staining showed decreased inflammatory cell infiltration in periodontitis tissue, while TRAP staining showed a decreased number of osteoclasts in the B10 transfer group compared to control group. The results suggest that B10 cells increased the production of SPMs, which may contribute to the inhibition of inflammatory cell infiltration and osteoclast differentiation in periodontitis.