Measuring the Effect of Methionine Deprivation in Genome Methylation of CCP Genes within the Prolaris Molecular Score Along Prostate Cancer Cell Lines
Abstract
Impairment of physiological metabolism is the trademark of various cancers and cancer cells as a component of their mechanism which ultimately bolsters survival. While normal cells are highly regulated and follow a sophisticated mechanism of cellular metabolism to achieve their goals of survival, cancer cells take the dysregulated path for survival, ultimately robbing the overall vital energies of the entire organism. Unfortunately, only a limited number of uniform ways exist that permit for a complete cure of various presentations of cancers. The PC3 cell line has been reported to be dependent upon exogenous pools of methionine, whereas the DU-145 cell line is partially independent. DU-145 satisfies its methionine requirements through the conversion of cysteine and homocysteine through a complex methionine recycling pathway. PC-3 and DU-145 cell lines were separately transfected with the Methionine Gamma Lyase Deaminase (Mgld) plasmid gene constructs and were expected to express the Mgld protein either within the cytoplasm or the nucleus. Essentially, this would degrade the endogenous methionine pools by availing gamma elimination and deamination. As a result, this leads us to question is what global metabolic effects would the Mgld have on the whole genome DNA methylation of any cancer cells? We sequenced the promoter and gene methylation and evaluated the differences of various transfectants. The primary focus was on the key enzymes that make up the Prolaris Molecular Score, specifically the 31-cell cycle progression (CCP) genes found within the tumor which are availed as indicators that measures the rate of prostate cancer tumor growth.
Faculty Sponsors
Dr. K.V. Venkatachalam
Project Type
Event
Location
Alvin Sherman Library
Start Date
4-6-2022 12:00 PM
End Date
4-7-2022 5:00 PM
Measuring the Effect of Methionine Deprivation in Genome Methylation of CCP Genes within the Prolaris Molecular Score Along Prostate Cancer Cell Lines
Alvin Sherman Library
Impairment of physiological metabolism is the trademark of various cancers and cancer cells as a component of their mechanism which ultimately bolsters survival. While normal cells are highly regulated and follow a sophisticated mechanism of cellular metabolism to achieve their goals of survival, cancer cells take the dysregulated path for survival, ultimately robbing the overall vital energies of the entire organism. Unfortunately, only a limited number of uniform ways exist that permit for a complete cure of various presentations of cancers. The PC3 cell line has been reported to be dependent upon exogenous pools of methionine, whereas the DU-145 cell line is partially independent. DU-145 satisfies its methionine requirements through the conversion of cysteine and homocysteine through a complex methionine recycling pathway. PC-3 and DU-145 cell lines were separately transfected with the Methionine Gamma Lyase Deaminase (Mgld) plasmid gene constructs and were expected to express the Mgld protein either within the cytoplasm or the nucleus. Essentially, this would degrade the endogenous methionine pools by availing gamma elimination and deamination. As a result, this leads us to question is what global metabolic effects would the Mgld have on the whole genome DNA methylation of any cancer cells? We sequenced the promoter and gene methylation and evaluated the differences of various transfectants. The primary focus was on the key enzymes that make up the Prolaris Molecular Score, specifically the 31-cell cycle progression (CCP) genes found within the tumor which are availed as indicators that measures the rate of prostate cancer tumor growth.
