Abstract
Stem cells has been well characterized to be multipotent cells that can differentiate into multiple tissueforming cell lineages, as osteloblasts, adipocytes, chondrocytes, tenocytes and myocytes. It has been shown that bone marrow derived cells provide stem and progenitor cells to wounds during inflammation, proliferative and remodeling phase of wound healing. On the other hand, mice have become the premier animal model for the study of numerous complex human diseases. Mouse models are cost effective and have a favorable generation time for cross breeding experiments. In our work we show the use of two mice models to determine which is the most applicable to evidence the function of stem cells during wound healing. Using the appropriate animal model, the mobilization and engraftment of different populations of stem cells (bone marrow complete cells, fresh bone marrow cell and mesenchymal stem cells) are analyzed by histology and flow cytometry, we will study the role of stem cells during repair of chronic wounds. This will further reveal what cells present in bone marrow contribute the most to the wound healing process.
Faculty Sponsors
Dr. James R. Munoz
Project Type
Event
Location
Alvin Sherman Library
Start Date
4-13-2012 1:00 PM
End Date
4-13-2012 5:30 PM
Role of Mesenchymal Stem Cells in Chronic Wound Healing
Alvin Sherman Library
Stem cells has been well characterized to be multipotent cells that can differentiate into multiple tissueforming cell lineages, as osteloblasts, adipocytes, chondrocytes, tenocytes and myocytes. It has been shown that bone marrow derived cells provide stem and progenitor cells to wounds during inflammation, proliferative and remodeling phase of wound healing. On the other hand, mice have become the premier animal model for the study of numerous complex human diseases. Mouse models are cost effective and have a favorable generation time for cross breeding experiments. In our work we show the use of two mice models to determine which is the most applicable to evidence the function of stem cells during wound healing. Using the appropriate animal model, the mobilization and engraftment of different populations of stem cells (bone marrow complete cells, fresh bone marrow cell and mesenchymal stem cells) are analyzed by histology and flow cytometry, we will study the role of stem cells during repair of chronic wounds. This will further reveal what cells present in bone marrow contribute the most to the wound healing process.
