Adhesion of Lysozyme to FDA Group II Contact Lenses and Vials

Adhesion of Lysozyme to FDA Group II Contact Lenses and Vials

Alvin Sherman Library 1054

Lysozyme is the major protein adhering to contact lenses. Lysozyme adhesion to contact lenses and glass vials was examined with bicinchoninic acid (BCA). Contact lenses were placed in vials on day 0, and some lenses were removed every day thereafter for five days. The concentration of lysozyme in the vials, and its adhesion to the lenses, was monitored daily. Lysozyme adhesion to FDA Group II lenses showed a cyclical pattern, with high levels of adhesion after 1 and 3 days of incubation, and lower levels of adhesion after 2 and 4 days of incubation. These differences were all statistically significant (P < 0.05), and reflect both adhesion and de-adhesion processes occurring as the incubation proceeded. The concentration of lysozyme in the vials was not different after four days of incubation, compared to the start of the experiment (P > 0.05).