Document Type
Article
Publication Date
10-1-2014
Publication Title
Hypertension
Keywords
angiotensin II, angiotensin receptor blockers, angiotensin-converting enzyme 2, baroreflex
ISSN
0194-911X
Volume
64
Issue/No.
4
First Page
777
Last Page
83
Abstract
Angiotensin II increases and decreases arterial pressure by acting at angiotensin type 1 and type 2 receptors, respectively. Renovascular hypertensive rats exhibit a high level of activity of the peripheral and central renin-angiotensin system. Therefore, in the present study, we evaluated the effect of increasing the expression of angiotensin type 2 receptors in the solitary-vagal complex (nucleus of the solitary tract/dorsal motor nucleus of the vagus), a key brain stem region for cardiovascular regulation, on the development of renovascular hypertension. Holtzman normotensive rats were implanted with a silver clip around the left renal artery to induce 2-kidney 1-clip renovascular hypertension. Three weeks later, rats were microinjected in the solitary-vagal complex with either an adenoassociated virus to increase the expression of angiotensin type 2 receptors or with a control vector. We observed that increasing angiotensin type 2 receptor expression in the solitary-vagal complex attenuated the development of renovascular hypertension and also reversed the impairment of the baroreflex and the increase in the low-frequency component of systolic blood pressure observed in renovascular hypertensive rats. Furthermore, an observed decrease in mRNA levels of angiotensin-converting enzyme 2 in the solitary-vagal complex of renovascular hypertensive rats was restored to control levels after viral-mediated increases in angiotensin type 2 receptors at this site. Collectively, these data demonstrate specific and beneficial effects of angiotensin type 2 receptors via the brain of hypertensive rats and suggest that central angiotensin type 2 receptors may be a potential target for therapeutics in renovascular hypertension.
NSUWorks Citation
Blanch, Graziela Torres; Freiria-Oliveira, André Henrique; Speretta, Guilherme Fleury; Carrera, Eduardo J.; Li, Hongwei; Speth, Robert C.; Colombari, Eduardo; Sumners, Colin; and Colombari, Débora S., "Increased expression of angiotensin II type 2 receptors in the solitary-vagal complex blunts renovascular hypertension" (2014). HPD Articles. 88.
https://nsuworks.nova.edu/hpd_facarticles/88
ORCID ID
0000-0002-6434-2154
DOI
10.1161/HYPERTENSIONAHA.114.03188
Copyright
© 2014 American Heart Association, Inc.
