Intracerebral adeno-associated virus gene delivery of apolipoprotein E2 markedly reduces brain amyloid pathology in Alzheimer's disease mouse models
Document Type
Article
Publication Date
8-1-2016
Publication Title
Neurobiology of Aging
Keywords
Adeno-associated virus, Alzheimer's disease, Amyloid pathology, Apolipoprotein E2, Gene delivery
ISSN
0197-4580
Volume
44
First Page
159
Last Page
172
Abstract
The common apolipoprotein E alleles (ε4, ε3, and ε2) are important genetic risk factors for late-onset Alzheimer's disease, with the ε4 allele increasing risk and reducing the age of onset and the ε2 allele decreasing risk and markedly delaying the age of onset. Preclinical and clinical studies have shown that apolipoprotein E (APOE) genotype also predicts the timing and amount of brain amyloid-β (Aβ) peptide deposition and amyloid burden (ε4 >ε3 >ε2). Using several administration protocols, we now report that direct intracerebral adeno-associated virus (AAV)-mediated delivery of APOE2 markedly reduces brain soluble (including oligomeric) and insoluble Aβ levels as well as amyloid burden in 2 mouse models of brain amyloidosis whose pathology is dependent on either the expression of murine Apoe or more importantly on human APOE4. The efficacy of APOE2 to reduce brain Aβ burden in either model, however, was highly dependent on brain APOE2 levels and the amount of pre-existing Aβ and amyloid deposition. We further demonstrate that a widespread reduction of brain Aβ burden can be achieved through a single injection of vector via intrathalamic delivery of AAV expressing APOE2 gene. Our results demonstrate that AAV gene delivery of APOE2 using an AAV vector rescues the detrimental effects of APOE4 on brain amyloid pathology and may represent a viable therapeutic approach for treating or preventing Alzheimer's disease especially if sufficient brain APOE2 levels can be achieved early in the course of the disease.
NSUWorks Citation
Zhao, Lingzhi; Gottesdiener, Andrew J.; Parmar, Mayur; Li, Mingjie; Kaminsky, Stephen M.; Chiuchiolo, Maria J.; Sondhi, Dolan; Sullivan, Patrick M.; Holtzman, David M.; Crystal, Ronald G.; and Paul, Steven M., "Intracerebral adeno-associated virus gene delivery of apolipoprotein E2 markedly reduces brain amyloid pathology in Alzheimer's disease mouse models" (2016). HPD Articles. 23.
https://nsuworks.nova.edu/hpd_facarticles/23
ORCID ID
0000-0003-4970-9857
DOI
10.1016/j.neurobiolaging.2016.04.020
Copyright
Copyright © 2016 Elsevier Inc. All rights reserved.
