Student Theses, Dissertations and Capstones

Document Type

Thesis - NSU Access Only

Degree Name

Master of Science (M.S.) in Dentistry

Copyright Statement

All rights reserved. This publication is intended for use solely by faculty, students, and staff of Nova Southeastern University. No part of this publication may be reproduced, distributed, or transmitted in any form or by any means, now known or later developed, including but not limited to photocopying, recording, or other electronic or mechanical methods, without the prior written permission of the author or the publisher.

Department

College of Dental Medicine

First Advisor

Thyagseely Premaraj

Date of original Performance / Presentation

January 2023

Publication Date / Copyright Date

1-1-2023

Abstract

Objective: To determine if there is an induction of inflammation in OBA-9, PDL, THP-1, and hGMSC cells upon exposure to Invisalign® SmartTrackTM material elute. Methods Invisalign® trays were soaked in Phosphate buffered (PBS) solution for a period of 3 days, 1 week, 2 weeks, and 4 weeks. The solution was then collected and added to In-vitro grown oral epithelial (OBA-9) cells, Periodontal Ligament (PDL) cells, THP-1 Cell Line (Monocyte), and Human Gingival Mesenchymal Stem Cells (hGMSCs). Each cell was cultured in its appropriate medium according to specific cell culture protocols. Invisalign® elute and PBS (control) were added at different concentrations to the cells. After 24 hours, the cells were tested for toxicity using water-soluble tetrazolium salt (WST-1) assay. Furthermore, pro-inflammatory cytokine release of Interleukin-1β (IL-1β) was profiled using enzyme- linked immunosorbent assay (ELISA). Each experiment was repeated three times and statistical analysis was done accordingly. Results: Our findings demonstrated a time-dependent release of IL-1β upon exposure to Invisalign® elute to THP-1, OBA-9, and PDL cells, while no distinguishable pattern was determined for hGMSCs. The release of IL-1β was not statistically significant when compared to control (p>0.05). However, the WST-1 cell viability assay revealed that lower concentrations of the elute produced little to no cytotoxicity, while higher concentrations of elute resulted in moderate to severe cytotoxicity. Conclusion: There was some evidence to suggest that SmartTrackTM material induces inflammation. Our results showed an increase release of IL-1β upon exposure to higher concentration of elute, however, no statistical significance was found. Monocytes, epithelial cells, and periodontal ligament cells showed an increased cell death upon exposure to higher concentration of SmartTrackTM elute, indicating cell cytotoxicity. Our study suggest that SmartTrackTM material induces cell death and promotes the release of inflammatory cytokine, IL-1β. However, interpreting in-vitro effects on patient care should be exercised cautiously. More research is needed to elucidate the mechanism of cell death and clinical effects on patients.

Disciplines

Dentistry

Keywords

adverse effects; IL-1B; Inflammation; Interleukin 1; Invisalign

Download

Download Full Text (1.7 MB)

Download
To access this thesis/dissertation you must have a valid nova.edu OR mynsu.nova.edu email address and create an account for NSUWorks.

Free My Thesis

If you are the author of this work and would like to grant permission to make it openly accessible to all, please click the Free My Thesis button.

  Contact Author

Share

 
COinS