Student Theses, Dissertations and Capstones

Document Type

Thesis - NSU Access Only

Degree Name

Master of Science (M.S.) in Dentistry

Copyright Statement

All rights reserved. This publication is intended for use solely by faculty, students, and staff of Nova Southeastern University. No part of this publication may be reproduced, distributed, or transmitted in any form or by any means, now known or later developed, including but not limited to photocopying, recording, or other electronic or mechanical methods, without the prior written permission of the author or the publisher.

Department

College of Dental Medicine

First Advisor

Xiaozhe Han

Date of original Performance / Presentation

January 2024

Publication Date / Copyright Date

1-1-2024

Abstract

Introduction: Periodontitis is a chronic inflammatory disease that involves inflammation in the attachment apparatus. Patients with aggressive forms of the disease respond less to therapy, and their treatment often aims for rehabilitation. The key to understand the lack of response is to study what happens on the cellular level locally and systemically and identify the immuno-cellular response in this specific group when compared to subjects with mild forms of the disease. Methods: In this cross-sectional study, six gingival tissue samples were collected from periodontitis patients with mild-moderate forms (Grade A/B) and severe forms (Grade C) of disease progression. The samples were processed through immune-inflammatory assay, Rt-qPCR and RNA sequencing to determine the difference in behavior between the two profiles. Results: On a systemic level, results from the human inflammation assay show a non-significant difference between the two groups in relation to the anti-inflammatory cytokines and a significant difference between the two groups for the pro-inflammatory results. On a local level, Rt-qPCR showed a significant difference in the level of expression of IL-10, being highly expressed in the A/B group. Results from the RNA sequencing shows upregulation of the proinflammatory biomarkers in the C group. Conclusions: A distinct local and systemic immune profile was observed in Grade C compared to grade A/B patients, particularly cellular and molecular events involving IL-10 pathway signaling.

Disciplines

Dentistry

Keywords

Host response; Immune response; Periodontitis; Periodontium

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