A subset of human rapidly self-renewing marrow stromal cells preferentially engraft in mice

Author(s)

R.H. Lee
S.C. Hsu
James R. Munoz, Nova Southeastern UniversityFollow
J.S. Jung
N.R. Lee
R. Pochampally
D.J. Prockop

Document Type

Article

Publication Title

Blood

ISSN

0006-4971

Publication Date

1-1-2006

Abstract

Controversies have arisen as to whether adult stem cells or progenitor cells from bone marrow can engraft into nonhematopoietic tissues in vivo. To resolve some of the controversies, we developed a highly sensitive polymerase chain reaction-based single nucleotide polymorphism (PCR-SNP) assay for competitive engraftment of mixtures of stem/progenitor cells. We used the assay to follow engraftment in immunodeficient mice of subpopulations of the stem/progenitor cells from human bone marrow referred to as either mesenchymal stem cells or marrow stromal cells (MSCs). The engraftment into adult mice without induced tissue injury was low and variable, but there was preferential engraftment of a subpopulation of rapidly self-renewing MSCs (RS-MSCs) compared with a subpopulation of slowly renewing MSCs (SR-MSCs). After intravenous infusion, there was a tendency for the cells to engraft into the hippocampal region that was previously designated a "vascular niche." Migration assays suggested that preferential engraftment of RS-MSCs was in part explained by their expression of CXCR4 and CX3R1, the receptors for SDF-1 and fractalkine.

DOI

10.1182/blood-2005-07-2701

Volume

107

Issue

5

First Page

2153

Last Page

2161

NSUWorks Citation

Lee, R., Hsu, S., Munoz, J. R., Jung, J., Lee, N., Pochampally, R., Prockop, D. (2006). A subset of human rapidly self-renewing marrow stromal cells preferentially engraft in mice. Blood, 107(5), 2153-2161.
Available at: https://nsuworks.nova.edu/cps_facarticles/1150