Faculty Articles

Early loss of renal transcripts in kidney allografts: relationship to morphologic changes and alloimmune effector mechanisms

Document Type

Article

Publication Title

PLoS Computational Biology

ISSN

1600-6135

Publication Date

1-1-2007

Abstract

We sought to understand the epithelial response to the T-cell mediated inflammatory process in kidney allograft rejection. Using microarrays, we studied transcriptome changes of kidney parenchymal cells and their relationship to the development of pathologic lesions such as tubulitis in mouse kidney allografts and isografts. Inflammatory infiltrate in allografts developed by day 5, but tubulitis first appeared at day 7 and was severe by day 21. Using microarrays, we selected 70 solute carrier transcripts with high renal parenchymal expression and known epithelial function. Transcript expression was reduced early in isografts and allografts, followed by progressive loss in allografts and recovery in isografts. The expression pattern of day 21 allografts developed progressively from the time of engraftment and was established before histologic lesions. These changes are probably functionally significant: selected proteins showed decreased immunostaining at days 7 and 21. Allospecific loss of transcripts was dependent on T cells but independent of perforin, granzymes A/B, CD103, or B cells. Weighted sum decomposition revealed multiple components of the epithelial response with allospecific changes from day 1. We conclude that loss of renal transcripts indicates an early stage of T-cell mediated alloimmune injury that later progresses to pathologic lesions such as tubulitis

DOI

10.1111/j.1600-6143.2007.01797.x

Volume

7

Issue

5

First Page

1121

Last Page

1130

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