A P450 Metabolism Experiment for Undergraduate Biochemistry Laboratories

Authors

Reza Razeghifard, Nova Southeastern UniversityFollow
Catherine Chiafair, Nova Southeastern UniversityFollow
Dimitri Giarikos, Nova Southeastern UniversityFollow

Document Type

Article

Publication Title

Journal of Chemical Education

ISSN

0021-9584

Publication Date

2014

Keywords

Upper-Division Undergraduate, Biochemistry, Laboratory Instruction, Collaborative/Cooperative Learning, Bioorganic Chemistry, Qualitative Analysis, Enzymes, Gas Chromatography, Mass Spectrometry, Quantitative Analysis

Abstract

A laboratory experiment is described to provide students hands-on experience in learning some aspects of microsomal P450-catalyzed metabolism. Undergraduate students in the biochemistry laboratories detect and quantify the metabolites produced from butylated hydroxytoluene (BHT) by liver microsomes using gas chromatography–mass spectrometry (GC–MS) techniques. The laboratory provides training to students for handling active microsomes, sample cleanup of biological matrices, extraction of organic metabolites, GC–MS analysis, data interpretation of complex mixtures, and collaborative work.

DOI

10.1021/ed400425d

Volume

91

Issue

1

First Page

141

Last Page

144

Comments

©2013 The American Chemical Society and Division of Chemical Education, Inc.

NSUWorks Citation

Razeghifard, R., Chiafair, C., & Giarikos, D. (2014). A P450 Metabolism Experiment for Undergraduate Biochemistry Laboratories. Journal of Chemical Education, 91, (1), 141 - 144. https://doi.org/10.1021/ed400425d. Retrieved from https://nsuworks.nova.edu/cnso_chemphys_facarticles/71