"Glycosylation Modulates Melanoma Cell α2β1 and α3β1 Integrin Interacti" by Maciej J. Stawikowski, Beatrix Aukszi et al.

Chemistry and Physics Faculty Articles

Title

Glycosylation Modulates Melanoma Cell α2β1 and α3β1 Integrin Interactions with Type IV Collagen

Authors

Maciej J. Stawikowski, Torrey Pines Institute for Molecular Studies
Beatrix Aukszi, Nova Southeastern UniversityFollow
Roma Stawikowska, Torrey Pines Institute for Molecular Studies
Mare Cudic, Nova Southeastern University
Gregg B. Fields, Torrey Pines Institute for Molecular Studies

Document Type

Article

Publication Date

6-23-2014

Publication Title

Journal of Biological Chemistry

Keywords

Collagen, Glycosylation, Hydroxylysine(Hyl), Integrins, Melanoma, Triple-helix

ISSN

0021-9258

Volume

289

Issue/No.

First Page

21591

Last Page

21604

Abstract

Although type IV collagen is heavily glycosylated, the influence of this posttranslational modification on integrin binding has not been investigated. In the present study, galactosylated and non-galactosylated triple-helical peptides have been constructed containing the α1(IV)382-393 and α1(IV)531-543 sequences, which are binding sites for the α2β1 and α3β1 integrins, respectively. All peptides had triple-helical stabilities of 37 °C or greater. The galactosylation of Hyl393 in α1(IV)382-393 and Hyl540 and Hyl543 in α1(IV)531-543 had a dose dependent influence on melanoma cell adhesion which was much more pronounced in the case of α3β1 integrin binding. Molecular modeling indicated that galactosylation occurred on the periphery of α2β1 integrin interaction with α1(IV)382-393 but right in the middle of α3β1 integrin interaction with α1(IV)531-543. The possibility of extracellular deglycosylation of type IV collagen was investigated, but no β-galactosidase-like activity capable of collagen modification was found. Thus, glycosylation of collagen can modulate integrin binding, and levels of glycosylation could be altered by reduction in expression of glycosylation enzymes but most likely not by extracellular deglycosylation activity.

Comments

NSUWorks Citation

Stawikowski, M. J., Aukszi, B., Stawikowska, R., Cudic, M., & Fields, G. B. (2014). Glycosylation Modulates Melanoma Cell α2β1 and α3β1 Integrin Interactions with Type IV Collagen. Journal of Biological Chemistry, 289, (31), 21591 - 21604. https://doi.org/10.1074/jbc.M114.572073. Retrieved from https://nsuworks.nova.edu/cnso_chemphys_facarticles/6

DOI

10.1074/jbc.M114.572073

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