Title

Comparative Anchor Tagged Sequences (CATS) for Integrative Mapping of Mammalian Genomes

Authors

Leslie A. Lyons, National Cancer Institute at Frederick
Thomas F. Laughlin, National Cancer Institute at Frederick
Neal G. Copeland, National Cancer Institute at Frederick
Nancy A. Jenkins, National Cancer Institute at Frederick
James E. Womack, Texas A&M University - College Station
Stephen J. O'Brien, National Cancer Institute at FrederickFollow

Document Type

Article

Publication Date

1-1997

Publication Title

Nature Genetics

ISSN

1061-4036

Volume

15

Issue/No.

1

First Page

47

Last Page

56

Abstract

Precise comparisons of mammalian gene maps require common anchor loci as landmarks for conserved chromosomal segments. Using a computer script that automates DNA sequence database alignments, we designed 410 evolutionary conserved primer pair sequences which are specific for anchor locus gene amplification from any mammalian species' DNA. Primer pairs were designed to span introns for polymorphism ascertainment, and to include sufficient exonic sequence (25−400 bp) to allow for gene identification. A total of 318 primer pairs were optimized for domestic cats, and 86% of the sequenced feline PCR products showed homology to the gene of primer origin. A screen of 20 mammals from 11 orders revealed that 35−52% of the 318 primers yielded a single PCR product without further optimization suggesting that nearly 75% can be optimized for any eutherian mammal.

Comments

©1997 Nature Publishing Group

NSUWorks Citation

Lyons, Leslie A.; Thomas F. Laughlin; Neal G. Copeland; Nancy A. Jenkins; James E. Womack; and Stephen J. O'Brien. 1997. "Comparative Anchor Tagged Sequences (CATS) for Integrative Mapping of Mammalian Genomes." Nature Genetics 15, (1): 47-56. https://nsuworks.nova.edu/cnso_bio_facarticles/699

ORCID ID

0000-0001-7353-8301

ResearcherID

N-1726-2015