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Biology Faculty Articles
Title
Automated Single-Strand Conformation Polymorphism Reveals Low Diversity of a Major Histocompatibility Complex Class II Gene in the Threatened New Zealand Sea Lion
Document Type
Article
Publication Date
9-2003
Publication Title
Molecular Ecology Resources
Keywords
ABI, DQB, Genetic diversity, Major Histocompatibility Complex, New Zealand sea lion, Population genetics, SSCP
ISSN
1755-098X
Volume
3
Issue/No.
2
First Page
346
Last Page
349
Abstract
Single-strand conformation polymorphism (SSCP) analysis of the second exon of the DQB gene in the endemic New Zealand sea lion (Phocarctos hookeri) was automated using fluorescently-labelled PCR primers and a capillary sequencer. SSCP mobility profiles (n = 61 individuals) were confirmed by direct sequencing of genomic polymerase chain reaction (PCR) products (n = 39). We found only two alleles defined by changes at a single codon of this apparently functional locus. SSCP at a constant temperature above ambient allowed consistent and rapid genotyping. By comparison, sequence-based genotyping was highly dependent on the threshold used to identify secondary peaks indicative of a heterozygote.
NSUWorks Citation
Lento, G. M.; C. S. Baker; Victor David; Naoya Yuhki; N. J. Gales; and Stephen J. O'Brien. 2003. "Automated Single-Strand Conformation Polymorphism Reveals Low Diversity of a Major Histocompatibility Complex Class II Gene in the Threatened New Zealand Sea Lion." Molecular Ecology Resources 3, (2): 346-349. https://nsuworks.nova.edu/cnso_bio_facarticles/602
ORCID ID
0000-0001-7353-8301
ResearcherID
N-1726-2015
Comments
©2003 Blackwell Publishing Ltd