Isolation and Characterization of Microsatellite Markers in Pangolins (Mammalia, Pholidota, Manis spp.)

Authors

• Shu-Jin Luo, National Cancer Institute at Frederick; University of Minnesota - St. Paul
• Qing-xiu Cai, Beijing Normal University - China
• Victor A. David, National Cancer Institute at Frederick
• Li Zhang, Beijing Normal University - China
• Paolo Martelli, Ocean Park - Hong Kong, China
• Norman T-L. Lim, National University of Singapore
• Nuno Ferrand, Universidade do Porto - Portugal
• Shih-Chien Chin, Taipei Zoo - Taiwan
• Philippe Gaubert, Institut de Recherche pour le Developpement - France
• Maria J. Ramos, Universidade do Porto - Portugal
• Stephen J. O'Brien, National Cancer Institute at FrederickFollow
• Agostinho Antunes, National Cancer Institute at Frederick; Universidade do Porto
• Warren E. Johnson, National Cancer Institute at Frederick

ORCID

0000-0001-7353-8301

ResearcherID

N-1726-2015

Document Type

Article

Publication Title

Molecular Ecology Notes

ISSN

1471-8278

Publication Date

3-2007

Keywords

Manis, Microsatellite, Pangolin, Pholidota

Abstract

Thirty-four polymorphic dinucleotide microsatellite loci were developed in the Malayan pangolin Manis javanica. Of the 34 markers, 32 and 18 were also amplified, respectively, in the Chinese pangolin (Manis pentadactyla) and the African tree pangolin (Manis tricuspis). Analysis of 24 Malayan, 12 Chinese and 2 African tree pangolins showed high levels of variability (heterozygosity ranging from 0.321 to 0.708). These are the first available microsatellite markers in Pholidota and will be an invaluable tool for evolutionary and conservation genetic studies in pangolins.

Volume

7

Issue

2

First Page

269

Last Page

272

Comments

©2006 The Authors

Additional Comments

National Cancer Institute contract #: N01-CO-12400; Portuguese Foundation for Science and Technology grant #: BSE/47559/2002; National Geographic Society grant #: 7483-03; GenBank accession #s: DQ886446-DQ886479

NSUWorks Citation

Luo, Shu-Jin; Qing-xiu Cai; Victor A. David; Li Zhang; Paolo Martelli; Norman T-L. Lim; Nuno Ferrand; Shih-Chien Chin; Philippe Gaubert; Maria J. Ramos; Stephen J. O'Brien; Agostinho Antunes; and Warren E. Johnson. 2007. "Isolation and Characterization of Microsatellite Markers in Pangolins (Mammalia, Pholidota, Manis spp.)." Molecular Ecology Notes 7, (2): 269-272. https://nsuworks.nova.edu/cnso_bio_facarticles/505