Development of Y Chromosome Intraspecific Polymorphic Markers in the Felidae

Authors

• Shu-Jin Luo, National Cancer Institute at Frederick; University of Minnesota - St. Paul
• Warren E. Johnson, National Cancer Institute at Frederick
• Victor A. David, National Cancer Institute at Frederick
• Marilyn Menotti-Raymond, National Cancer Institute at Frederick
• R. Stanyon, University of Florence - Italy
• Qing-xiu Cai, Beijing Normal University - China
• Thomas W. Beck, National Cancer Institute at Frederick
• Naoya Yuhki, National Cancer Institute at Frederick
• Jill Pecon-Slattery, National Cancer Institute at Frederick
• James L. D. Smith, University of Minnesota- St. Paul
• Stephen J. O'Brien, National Cancer Institute at FrederickFollow

ORCID

0000-0001-7353-8301

ResearcherID

N-1726-2015

Document Type

Article

Publication Title

Journal of Heredity

ISSN

0022-1503

Publication Date

7-2007

Abstract

Y chromosome haplotyping based on microsatellites and single nucleotide polymorphisms (SNPs) has proved to be a powerful tool for population genetic studies of humans. However, the promise of the approach is hampered in the majority of nonhuman mammals by the lack of Y-specific polymorphic markers. We were able to identify new male-specific polymorphisms in the domestic cat Felis catus and 6 additional Felidae species with a combination of molecular genetic and cytogenetic approaches including 1) identifying domestic cat male-specific microsatellites from markers generated from a male cat microsatellite-enriched genomic library, a flow-sorted Y cosmid library, or a Y-specific cat bacteria artificial chromosome (BAC) clone, (2) constructing microsatellite-enriched libraries from flow-sorted Y chromosomes isolated directly from focal wildcat species, and (3) screening Y chromosome conserved anchored tagged sequences primers in Felidae species. Forty-one male-specific microsatellites were identified, but only 6 were single-copy loci, consistent with the repetitive nature of the Y chromosome. Nucleotide diversity (π) of Y-linked intron sequences (2.1 kbp) was in the range of 0 (tiger) to 9.95 × 10⁻⁴ (marbled cat), and the number of SNPs ranged from none in the tiger to 7 in the Asian leopard cat. The Y haplotyping system described here, consisting of 4 introns (SMCY3,SMCY7, UTY11, and DBY7) and 1 polymorphic microsatellite (SMCY-STR), represents the first available markers for tracking intraspecific male lineage polymorphisms in Felidae species and promises to provide significant insights to evolutionary and population genetic studies of the species.

Volume

98

Issue

5

First Page

400

Last Page

413

Comments

©The American Genetic Association. 2007. All rights reserved.

Additional Comments

National Cancer Institute contract #: N01-CO-12400

NSUWorks Citation

Luo, Shu-Jin; Warren E. Johnson; Victor A. David; Marilyn Menotti-Raymond; R. Stanyon; Qing-xiu Cai; Thomas W. Beck; Naoya Yuhki; Jill Pecon-Slattery; James L. D. Smith; and Stephen J. O'Brien. 2007. "Development of Y Chromosome Intraspecific Polymorphic Markers in the Felidae." Journal of Heredity 98, (5): 400-413. https://nsuworks.nova.edu/cnso_bio_facarticles/500