Biology Faculty Articles
Document Type
Article
Publication Date
1983
Publication Title
Yale Journal of Biology and Medicine
ISSN
0044-0086
Volume
56
Issue/No.
5-6
First Page
701
Last Page
708
Abstract
A summary of a survey of three genera of mycoplasmatales (Mycoplasma, Acholeplasma, and Ureaplasma) for isozyme expression is presented. Isozyme analysis of mycoplasmas has been employed in at least three distinct areas: (1) as genetic markers for identification, individualization, and taxonomic classification; (2) as markers for cell culture contamination; and (3) as a qualitative measure of the operative metabolic pathways in the diverse species. We have found five ubiquitous enzymes: purine nucleoside phosphorylase, adenylate kinase, inorganic pyrophosphatase, dipeptidase, and esterase. Three enzymes, glucose-6-phosphate dehydrogenase, phosphogluconate dehydrogenase, and superoxide dismutase, were restricted to Acholeplasma species and were not detected in Mycoplasma or Ureaplasma. Four glycolytic enzymes, glucose phosphate isomerase, triose phosphate isomerase, glyceraldehyde-3-phosphate dehydrogenase, and lactate dehydrogenase, were restricted to those species of Mycoplasma and Acholeplasma capable of glucose fermentation. Two of these glycolytic enzymes, glucose phosphate isomerase and lactate dehydrogenase, were detected in serovars I and II of U. urealyticum, which is inconsistent with the non-glycolytic activity in this genus.
NSUWorks Citation
O'Brien, Stephen J.; Janice M. Simonson; S. Razin; and Michael F. Barile. 1983. "On the Distribution and Characteristics of Isozyme Expression in Mycoplasma, Acholeplasma, and Ureaplasma Species." Yale Journal of Biology and Medicine 56, (5-6): 701-708. https://nsuworks.nova.edu/cnso_bio_facarticles/394
ORCID ID
0000-0001-7353-8301
ResearcherID
N-1726-2015
Comments
©1983 The Yale Journal of Biology and Medicine, Inc.