Title

Parallels of Genomic Organization and of Endogenous Retrovirus Organization in Cat and Man

Authors

Stephen J. O'Brien, National Cancer Institute at FrederickFollow
Roger Reeves, Johns Hopkins University
Janice M. Simonson, National Cancer Institute at Frederick
Mary Eichelberger, National Cancer Institute at Frederick
William G. Nash, National Cancer Institute at Frederick

Document Type

Article

Publication Date

1983

Publication Title

Developmental Genetics

Keywords

Retrovirus, Chromosomal evolution, Feline genetics, Somatic cell genetics

ISSN

0192-253X

Volume

4

Issue/No.

4

First Page

341

Last Page

354

Abstract

A combination of technical advances (most notably heterologous cell fusion, high resolution G-banding, and molecular cloning) has contributed to an accelerated advance in genetic analysis in mammals. The present human genetic map contains over 400 gene assignments and the map is growing rapidly as each new molecular clone or immunological reagent is developed. In our laboratory, we have developed a panel of rodent X human somatic cell hybrids that have been utilized in chromosome assignment of several classes of genes including oncogenes (ras, raf) and endogenous human retroviral sequences (ERVL, 2, etc). Using similar techniques, a biochemical genetic map of the domestic cat has been derived. The cat has 19 chromosome pairs and, to date, 40 genes have been mapped to 16 linkage or syntenic groups. Comparison of linkage relationships between homologous enzymes has revealed a striking conversation of chromosomal linkage association between cat and man. A comparison of syntenically homologous, highly extended high resoultion G-banded chromosomes between the two mammalian families revealed that 20–25%, by length, of the human karyotype can be precisely aligned (chromomere to chromomere) between cats and man despite the evolutionary divergence of the species nearly 80 million years ago.

Moderately repetitive families of retrovirus-related DNAs exist within the feline and the human genomes. We have isolated molecular clones of several members of the feline RD-114 retrovirus family from a genomic library of normal cat cellular DNA. The endogenous sequences analyzed were similar to each other in that they were colinear with RD-114 proviral DNA, were bounded by long terminal redundancies, and conserved many restriction sites in the gag and pol regions. Several sequences were apparently deleted, relative to the previously characterized inducible RD-114 genome. The env regions of a number of endogenous RD-114 sequences examined were substantially deleted or diverged; a subset of these sequences contained information at the position of the env region that was not homologous to inducible RD-114. The RD-114 virogenes were dispersed to several cat chrosomes that were localized using a panel of rodent x cat somatic cell hybrids. A comparison of the genetic properties of endogenous human retroviral sequences revealed several similarities between the human and feline status of endogenous retroviruses.

Comments

© 1983 Wiley-Liss, Inc.

Based on a lecture delivered in September 1983, at the internaitonal confrence in biochemical and developmental genetics, kos, greece.

NSUWorks Citation

O'Brien, Stephen J.; Roger Reeves; Janice M. Simonson; Mary Eichelberger; and William G. Nash. 1983. "Parallels of Genomic Organization and of Endogenous Retrovirus Organization in Cat and Man." Developmental Genetics 4, (4): 341-354. doi:10.1002/dvg.1020040410.

ORCID ID

0000-0001-7353-8301

ResearcherID

N-1726-2015

DOI

10.1002/dvg.1020040410