Biology Faculty Articles
Title
Dynamic and Nonspecific Dispersal of Human T-Cell Leukemia/Lymphoma Virus Type-I Integration in Cultured Lymphoma Cells
Document Type
Article
Publication Date
10-15-1986
Publication Title
Virology
ISSN
0042-6822
Volume
154
Issue/No.
1
First Page
67
Last Page
75
Abstract
The progression of HTLV-I proviral integration over a 3-year period of in vitro culture was examined in two human lymphoma lines, Hut 102 and MJ. Using specific HTLV-I molecular clones and a Southern analysis at different cell passages, Hut 102 increased from 2 to 19 integrated proviral integrations while MJ increased to at least 25 different integrations by passage 43. During the progress of increased superinfection and novel integration in vitro some of the previous proviral integrations were lost from the cultures. The 19 integrations of late passage Hut 102 cells were shown to be dispersed to 19 different human chromosomes by analysis of 34 distinct rodent × Hut 102 somatic cell hybrids which segregated human chromosomes (and included proviral integrations) in different combinations. The two primary integrations in Hut 102 were located on human chromosomes 4 and 20, respectively. A similar pattern of nonspecific integration was observed in somatic cell hybrid analysis of the 25 proviral integrations of MJ. The dynamic infection-reintegration process in vitro revealed in these studies may confuse experimental verification of potential cis acting functions of HTLV-I in the as yet poorly understood mechanism of neoplastic transformation.
NSUWorks Citation
Seigel, Leonard J.; William G. Nash; Bernard J. Poiesz; Janet L. Moore; and Stephen J. O'Brien. 1986. "Dynamic and Nonspecific Dispersal of Human T-Cell Leukemia/Lymphoma Virus Type-I Integration in Cultured Lymphoma Cells." Virology 154, (1): 67-75. https://nsuworks.nova.edu/cnso_bio_facarticles/380
ORCID ID
0000-0001-7353-8301
ResearcherID
N-1726-2015
Comments
© 1986 Published by Elsevier Inc.