Biology Faculty Articles
Title
Mechanism of met Oncogene Activation
Document Type
Article
Publication Date
6-20-1986
Publication Title
Cell
ISSN
0092-8674
Volume
45
Issue/No.
6
First Page
895
Last Page
904
Abstract
The met oncogene activated in vitro by treatment of a human osteogenic sarcoma (HOS) cell line with N-methyl-N′-nitronitrosoguanidine (MNNG) is related to the tyrosine kinase gene family. Probes from the met oncogene locus recognize two distinct transcripts of 9.0 kb and 10.0 kb which are independently expressed in a cell-type-specific fashion. While the met proto-oncogene locus expresses the 9.0 kb RNA and maps to human chromosome 7q21–31, the locus expressing the 10.0 kb RNA, (tpr; translocated promoter region) maps to human chromosome 1. Both MNNG-HOS cells and met NIH 3T3 transformants express a novel 5.0 kb RNA which represents a hybrid transcript with 5′ sequences derived from tpr and 3′ sequences from the met proto-oncogene. Treating HOS cells in vitro with MNNG, a known clastogenic carcinogen, resulted in fusion of two chromosomally disparate loci, met and tpr, generating the active met oncogene.
NSUWorks Citation
Park, Morag; Michael Dean; Colin S. Cooper; Martin Schmidt; Stephen J. O'Brien; Donald G. Blair; and George F. Vande Woude. 1986. "Mechanism of met Oncogene Activation." Cell 45, (6): 895-904. https://nsuworks.nova.edu/cnso_bio_facarticles/377
ORCID ID
0000-0001-7353-8301
ResearcherID
N-1726-2015
Comments
©Cell 1986