Biology Faculty Articles
Title
A Glutaminase (Gls) Gene Maps to Mouse Chromosome 1, Rat Chromosome 9, and Human Chromosome 2
Document Type
Article
Publication Date
8-1989
Publication Title
Genomics
ISSN
0888-7543
Volume
5
Issue/No.
2
First Page
291
Last Page
297
Abstract
A rat cDNA clone encoding a portion of phosphate-activated glutaminase was used to identify DNA restriction fragment length polymorphisms (RFLPs) in sets of somatic cell hybrids and between wild-derived and inbred strains of mice. Segregation of rat and mouse chromosomes among somatic cell hybrids indicated assignment to rat chromosome 9 and mouse chromosome 1. Analysis of chromosome 1 alleles for several genes in an interspecific cross between Mus spretus and C3H/HeJ - gld/gld mice indicates that glutaminase can be positioned within 5.5 ± 2.0 cM proximal to Ctla-4. Similarly, human-hamster somatic cell hybrids were examined for RFLPs, and four human EcoRI restriction fragments were found to hybridize with the rat glutaminase probe. Two of these restriction fragments cosegregated and mapped to human chromosome 2 in a region that is syntenic with mouse chromosome 1 and rat chromosome 9.
Additional Comments
GenBank accession #: M22586
NSUWorks Citation
Mock, Beverly; Christine Kozak; Michael F. Seldin; Naomi Ruff; Lawrence D'Hoostelaere; Claude Szpirer; Hector Seuanez; Stephen J. O'Brien; and Carl Banner. 1989. "A Glutaminase (Gls) Gene Maps to Mouse Chromosome 1, Rat Chromosome 9, and Human Chromosome 2." Genomics 5, (2): 291-297. https://nsuworks.nova.edu/cnso_bio_facarticles/342
ORCID ID
0000-0001-7353-8301
ResearcherID
N-1726-2015
Comments
© 1989 Published by Elsevier Inc.