Title

The t(11;14)(p15;q11) in a T-Cell Acute Lymphoblastic Leukemia Cell Line Activates Multiple Transcripts, Including Ttg-1, a Gene Encoding a Potential Zinc Finger Protein

Authors

Elizabeth A. McGuire, Washington University School of Medicine - St. Louis
Richard D. Hockett, Washington University School of Medicine - St. Louis
Karen M. Pollock, Washington University School of Medicine - St. Louis
Marty F. Bartholdi, Los Alamos National Laboratory
Stephen J. O'Brien, National Cancer Institute at FrederickFollow
Stanley J. Korsmeyer, Washington University School of Medicine - St. Louis

Document Type

Article

Publication Date

5-1989

Publication Title

Molecular and Cellular Biology

ISSN

0270-7306

Volume

9

Issue/No.

5

First Page

2124

Last Page

2132

Abstract

Interchromosomal translocations within lymphoid neoplasms frequently involve the antigen receptor genes. We cloned the breakpoints of the t(11;14)(p15;q11) in a CD3-negative T-cell acute lymphoblastic leukemia cell line (RPMI 8402) in order to identify new genes potentially involved in T-cell neoplasia. An extensive comparison of both breakpoints and their germ line counterparts indicated that an inadvertant recombinase-mediated break at chromosome segment 11p15 recombined with the delta T-cell receptor at 14q11. The derivative 11 breakpoint resembles a coding joint in which 11p15 rather than a variable region was introduced 5' to a D_δ1D_δ2J_δ1 intermediate rearrangement. Conversely, the derivative 14 breakpoint corresponds to a signal joint between the 5' heptamer-spacer-nonamer recombinational signal of D_δ1 and an isolated heptamer at 11p15. Multiple, apparently distinct transcripts were found flanking both breakpoints of 8402. RNAs of 3.5, 4.4, 1.4, and 8.0 kilobases originating from either side of the derivative 14 breakpoint were highly expressed in 8402 compared with other cells. This suggests that this translocation deregulated multiple genes and provides the opportunity to assess any multifactorial contribution they may have to malignancy. We cloned and sequenced several cDNAs representing the 1.4-kilobase transcript (termed Ttg-1 [T-cell translocation gene 1]) from an 8402 library. The predicted protein of 156 amino acids contained two internal repeats which could potentially form zinc fingers.

Comments

©1989, American Society for Microbiology

Additional Comments

NIH grant #: RR01315; American Cancer Society grant #: PRTF-87

NSUWorks Citation

McGuire, Elizabeth A.; Richard D. Hockett; Karen M. Pollock; Marty F. Bartholdi; Stephen J. O'Brien; and Stanley J. Korsmeyer. 1989. "The t(11;14)(p15;q11) in a T-Cell Acute Lymphoblastic Leukemia Cell Line Activates Multiple Transcripts, Including Ttg-1, a Gene Encoding a Potential Zinc Finger Protein." Molecular and Cellular Biology 9, (5): 2124-2132. https://nsuworks.nova.edu/cnso_bio_facarticles/301

ORCID ID

0000-0001-7353-8301

ResearcherID

N-1726-2015