Biology Faculty Articles
Title
Mapping of PRM 1 to Human Chromosome 16 and Tight Linkage of Prm-1 and Prm-2 on Mouse Chromosome 16
Document Type
Article
Publication Date
9-1989
Publication Title
Journal of Heredity
ISSN
0022-1503
Volume
80
Issue/No.
6
First Page
442
Last Page
446
Abstract
The protamines are small, arginine-rich nuclear proteins that replace histones and transition proteins late in the haploid phase of spermatogenesis in mammals. The two mouse genes encoding protamines– Prm-1 and Prm-2– have been molecularly cloned and mapped to mouse chromosome 16 (MMU 16). A cDNA clone of mouse Prm-1 that hybridized to the corresponding human gene was used to analyze a panel of somatic cell hybrids made between human lymphoblasts and the E36 hamster cell line. The human gene, which we have designated PRM1, was syntenic with human chromosome 16 (HSA 16) and discordant with all other human chromosomes. Linkage analysis in the mouse was accomplished using the backcross (Czech II × BALB/c Pt) × Czech II to map Prm-1 and Prm-2 to a position near the 5’ terminus of MMU 16. No recombination between Prm-1 and Prm-2 was observed among 89 progeny of the Czech II × BALB/c cross or among 94 progeny of the backcross (CBA/J × BALB/cJ) × BALB/cJ, demonstrating that the two loci are separated by less than 1.6 cM on MMU 16. This tight linkage may be of functional significance, as Prm-1 and Prm-2 are among a limited number of genes known to be expressed postmeiotically in male haploid germ cells.
Additional Comments
Public Health Service Grant #s: PO1 HD19920, RO1 HD22262, GM29224
NSUWorks Citation
Reeves, Roger; John Gearhart; N. B. Hecht; P. Yelick; P. Johnson; and Stephen J. O'Brien. 1989. "Mapping of PRM 1 to Human Chromosome 16 and Tight Linkage of Prm-1 and Prm-2 on Mouse Chromosome 16." Journal of Heredity 80, (6): 442-446. https://nsuworks.nova.edu/cnso_bio_facarticles/299
ORCID ID
0000-0001-7353-8301
ResearcherID
N-1726-2015
Comments
©1990 The American Genetic Association