Molecular Cloning and Chromosomal Localization of the Human T-Cell Receptor ζ Chain: Distinction from the Molecular CD3 Complex

Authors

• Allan M. Weissman, National Institute of Child Health and Human Development
• Damon Hou, National Institute of Child Health and Human Development
• David G. Orloff, National Institute of Child Health and Human Development
• William S. Modi, National Cancer Institute at Frederick
• Hector Seuanez, National Cancer Institute at Frederick
• Stephen J. O'Brien, National Cancer Institute at FrederickFollow
• Richard D. Klausner, National Institute of Child Health and Human Development

ORCID

0000-0001-7353-8301

ResearcherID

N-1726-2015

Document Type

Article

Publication Title

Proceedings of the National Academy of Sciences of the United States of America

ISSN

1091-6490

Publication Date

12-15-1988

Keywords

cDNA, Receptor subunits, T-cell activation

Abstract

The T-cell antigen receptor (TCR) is a multisubunit receptor complex specific to T cells subserving both antigen recognition and signal transduction functions. The ζ chain of the TCR is a component of all surface receptor complexes. This chain was first identified in murine T cells by virtue of the fact that it coimmunoprecipitates with the TCR complex using antibodies directed against either the clonespecific subunits or invariant CD3 subunits of the receptor. Recently, we have isolated a cDNA encoding the murine ζ. Using this as a probe, we have now isolated cDNAs encoding the human ζ. Sequence analysis of cDNAs encoding human and murine ζ reveals that it is a highly conserved protein. In addition to amino acid homology, there is remarkable interspecies conservation in the nucleotide sequence of the 5' and 3' untranslated regions of the C mRNA. The previously characterized invariant �, ε, and γ chains of the TCR, referred to as the CD3 complex, share significant sequence and structural homology with each other and are all located within 300 kilobases of each other on human chromosome 11 (11q23). ζ has no sequence similarity to the CD3 chains and the localization of the human ζ gene to the centromeric region of chromosome 1 underscores the fact that it is a distinct genetic component of the TCR.

Volume

85

Issue

24

First Page

9709

Last Page

9713

NSUWorks Citation

Weissman, Allan M.; Damon Hou; David G. Orloff; William S. Modi; Hector Seuanez; Stephen J. O'Brien; and Richard D. Klausner. 1988. "Molecular Cloning and Chromosomal Localization of the Human T-Cell Receptor ζ Chain: Distinction from the Molecular CD3 Complex." Proceedings of the National Academy of Sciences of the United States of America 85, (24): 9709-9713. https://nsuworks.nova.edu/cnso_bio_facarticles/238