Biology Faculty Articles
Title
Gene for T-Cell Growth Factor: Location on Human Chromosome 4q and Feline Chromosome B1
Document Type
Article
Publication Date
1-13-1984
Publication Title
Science
ISSN
0036-8075
Volume
223
Issue/No.
4632
First Page
175
Last Page
178
Abstract
T-cell growth factor (TCGF) or interleukin-2 (IL-2), an immunoregulatory lymphokine, is produced by lectin- or antigen-activated mature T lymphocytes and in a constitutive manner by certain T-cell lymphoma cell lines. By means of a molecular clone of human TCGF and DNA extracted from a panel of somatic cell hybrids (rodent cells x normal human lymphocytes), the TCGF structural gene was identified on human chromosome 4. In situ hybridization of the TCGF clone to human chromosomes resulted in significant labeling of the midportion of the long arm of chromosome 4, indicating that the TCGF gene was located at band q26-28. Genomic DNA from a panel of hybrids prepared with HUT-102 B2 cells was examined with the same molecular clone. In this clone of cells, which produces human T-cell leukemia virus, the TCGF gene was also located on chromosome 4 and was apparently not rearranged. The homologous TCGF locus in the domestic cat was assigned to chromosome B1 by using a somatic cell hybrid panel that segregates cat chromosomes. Linkage studies as well as high-resolution G-trypsin banding indicate that this feline chromosome is partially homologous to human chromosome 4.
NSUWorks Citation
Seigel, Leonard J.; Mary E. Harper; Flossie Wong-Staal; Robert C. Gallo; William Nash; and Stephen J. O'Brien. 1984. "Gene for T-Cell Growth Factor: Location on Human Chromosome 4q and Feline Chromosome B1." Science 223, (4632): 175-178. doi:10.1126/science.6318318.
ORCID ID
0000-0001-7353-8301
ResearcherID
N-1726-2015
DOI
10.1126/science.6318318
Comments
©1984 American Association for the Advancement of Science