Biology Faculty Articles

Title

Automated Single-Strand Conformation Polymorphism Reveals Low Diversity of a Major Histocompatibility Complex Class II Gene in the Threatened New Zealand Sea Lion

Document Type

Article

Publication Date

9-2003

Publication Title

Molecular Ecology Resources

Keywords

ABI, DQB, Genetic diversity, Major Histocompatibility Complex, New Zealand sea lion, Population genetics, SSCP

ISSN

1755-098X

Volume

3

Issue/No.

2

First Page

346

Last Page

349

Abstract

Single-strand conformation polymorphism (SSCP) analysis of the second exon of the DQB gene in the endemic New Zealand sea lion (Phocarctos hookeri) was automated using fluorescently-labelled PCR primers and a capillary sequencer. SSCP mobility profiles (n = 61 individuals) were confirmed by direct sequencing of genomic polymerase chain reaction (PCR) products (n = 39). We found only two alleles defined by changes at a single codon of this apparently functional locus. SSCP at a constant temperature above ambient allowed consistent and rapid genotyping. By comparison, sequence-based genotyping was highly dependent on the threshold used to identify secondary peaks indicative of a heterozygote.

Comments

©2003 Blackwell Publishing Ltd

ORCID ID

0000-0001-7353-8301

ResearcherID

N-1726-2015

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