Biology Faculty Articles

Title

Dynamic and Nonspecific Dispersal of Human T-Cell Leukemia/Lymphoma Virus Type-I Integration in Cultured Lymphoma Cells

Document Type

Article

Publication Date

10-15-1986

Publication Title

Virology

ISSN

0042-6822

Volume

154

Issue/No.

1

First Page

67

Last Page

75

Abstract

The progression of HTLV-I proviral integration over a 3-year period of in vitro culture was examined in two human lymphoma lines, Hut 102 and MJ. Using specific HTLV-I molecular clones and a Southern analysis at different cell passages, Hut 102 increased from 2 to 19 integrated proviral integrations while MJ increased to at least 25 different integrations by passage 43. During the progress of increased superinfection and novel integration in vitro some of the previous proviral integrations were lost from the cultures. The 19 integrations of late passage Hut 102 cells were shown to be dispersed to 19 different human chromosomes by analysis of 34 distinct rodent × Hut 102 somatic cell hybrids which segregated human chromosomes (and included proviral integrations) in different combinations. The two primary integrations in Hut 102 were located on human chromosomes 4 and 20, respectively. A similar pattern of nonspecific integration was observed in somatic cell hybrid analysis of the 25 proviral integrations of MJ. The dynamic infection-reintegration process in vitro revealed in these studies may confuse experimental verification of potential cis acting functions of HTLV-I in the as yet poorly understood mechanism of neoplastic transformation.

Comments

© 1986 Published by Elsevier Inc.

ORCID ID

0000-0001-7353-8301

ResearcherID

N-1726-2015

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