Transactivation Induced by Human T-Lymphotropic Virus Type III (HTLV III) Maps to a Viral Sequence Encoding 58 Amino Acids and Lacks Tissue Specificity
The acquired immune deficiency syndrome (AIDS) retrovirus, HTLV-III/LAV, encodes a transacting factor which directly or indirectly stimulates the expression of genes linked to its LTR. To further dissect this phenomenon, we have cotransfected a biologically active molecular clone of HTLV-III and a recombinant plasmid containing an indicator gene, the bacterial gene for chloramphenicol acetyltransferase (CAT), under the control of the HTLV-III LTR. Amplified CAT activity was detected in both lymphoid cells and fibroblasts from a number of species in the presence of the proviral DNA. Deletion experiments confirm the previous assignment of the gene required for transactivation to a region immediately 5′ to the envelope gene, and further narrow down the critical functional domain to a coding sequence of 58 codons.
Seigel, Leonard J.; Lee Ratner; Steven F. Josephs; David Dersefi; Mark B. Feinberg; Gregory R. Reyes; Stephen J. O'Brien; and Flossie Wong-Staal. 1986. "Transactivation Induced by Human T-Lymphotropic Virus Type III (HTLV III) Maps to a Viral Sequence Encoding 58 Amino Acids and Lacks Tissue Specificity." Virology 148, (1): 226-231. http://nsuworks.nova.edu/cnso_bio_facarticles/379