Biology Faculty Articles

Title

Transactivation Induced by Human T-Lymphotropic Virus Type III (HTLV III) Maps to a Viral Sequence Encoding 58 Amino Acids and Lacks Tissue Specificity

Document Type

Article

Publication Date

1-15-1986

Publication Title

Virology

ISSN

0042-6822

Volume

148

Issue/No.

1

First Page

226

Last Page

231

Abstract

The acquired immune deficiency syndrome (AIDS) retrovirus, HTLV-III/LAV, encodes a transacting factor which directly or indirectly stimulates the expression of genes linked to its LTR. To further dissect this phenomenon, we have cotransfected a biologically active molecular clone of HTLV-III and a recombinant plasmid containing an indicator gene, the bacterial gene for chloramphenicol acetyltransferase (CAT), under the control of the HTLV-III LTR. Amplified CAT activity was detected in both lymphoid cells and fibroblasts from a number of species in the presence of the proviral DNA. Deletion experiments confirm the previous assignment of the gene required for transactivation to a region immediately 5′ to the envelope gene, and further narrow down the critical functional domain to a coding sequence of 58 codons.

Comments

© 1986 Published by Elsevier Inc.

ORCID ID

0000-0001-7353-8301

ResearcherID

N-1726-2015

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