Biology Faculty Articles

Title

Molecular Characterization and Genetic Mapping of Class I and Class II MHC Genes of the Domestic Cat

Document Type

Article

Publication Date

6-1988

Publication Title

Immunogenetics

ISSN

0093-7711

Volume

27

Issue/No.

6

First Page

414

Last Page

425

Abstract

The major histocompatibility complex (MHC) of the domestic cat has been poorly characterized to date, primarily because of numerous difficulties in the preparation of allotypic sera. We present here a comparative analysis of class I and class II genes in domestic cat populations using molecular probes of the MHC from man and mouse. The cat possesses a minimum of 20 class I loci and 5 class II genes per haploid genome. Class I genes of the domestic cat expressed limited restriction fragment length polymorphism. The average percent difference of the size of DNA fragments between individual cats was 9.0 %, a value five times lower than the value for mice, but comparable to the human DNA polymorphism level. Class I and class II genes were both genetically mapped to feline chromosome B2 using a panel of rodent x cat somatic cell hybrids. Since feline chromosomeB2 is syntenically homologous to human chromosome 6 and mouse chromosome 17, these results affirm the linkage conservation of the MHC-containing linkage group in the three mammalian orders.

Comments

©1988 Springer-Verlag

ORCID ID

0000-0001-7353-8301

ResearcherID

N-1726-2015

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