Investigating the Impact of Lipogenesis Inhibition on the Phospholipid Composition of Melanoma Cells Using High-Performance Liquid Chromatography Mass Spectrometry
Abstract
Melanoma, the most lethal variant of skin cancer, has a rate of incidence that continues to increase globally. The upregulation of lipogenesis is a primary feature of melanoma. However, the role of phospholipids (PLs) in the onset and progression of melanoma is not well-characterized. In this study we employed high-performance liquid chromatography mass spectrometry (HPLC-MS) to elucidate alterations in the phospholipidomic profile of human melanoma cells after inhibition of fatty acid synthase (FASN), a key enzyme in lipogenesis. Our results show that inhibition of FASN decreased cell viability by ~30%, underscoring the importance of lipogenesis for the survival of melanoma cells. HPLC-MS analyses of the cells showed that the relative abundance of phosphatidylcholine, phosphatidylethanolamine, and phosphatidylinositol PL classes increased, while phosphatidylglycerol (PG), phosphatidylserine, and sphingomyelin (SM) decreased. Furthermore, we determined variations in the composition of the individual molecular species within the PL classes as a function of FASN inhibition. This analysis led to the identification of specific PL molecular species such as 34:1-PG and 40:1-SM, which exhibited more than a ten percent change in their relative abundance upon FASN inhibition, suggesting potentially significant roles in lipogenesis and the survival of melanoma cells (individual PL molecular species are designated as the total number of carbon atoms in the FA chains (or FA chain and sphingosine):total number of double bonds in the FA chains (or FA chain and sphingosine)-name of the PL class). The results from this study offer initial insights into the function of PLs in melanoma pathogenesis.
Faculty Sponsors
Dr. Dmitriy Minond, Dr. Robert Speth, Dr. Richard H. Perry
Project Type
Event
Location
Alvin Sherman Library
Start Date
4-3-2024 12:30 PM
End Date
4-4-2024 1:30 PM
Investigating the Impact of Lipogenesis Inhibition on the Phospholipid Composition of Melanoma Cells Using High-Performance Liquid Chromatography Mass Spectrometry
Alvin Sherman Library
Melanoma, the most lethal variant of skin cancer, has a rate of incidence that continues to increase globally. The upregulation of lipogenesis is a primary feature of melanoma. However, the role of phospholipids (PLs) in the onset and progression of melanoma is not well-characterized. In this study we employed high-performance liquid chromatography mass spectrometry (HPLC-MS) to elucidate alterations in the phospholipidomic profile of human melanoma cells after inhibition of fatty acid synthase (FASN), a key enzyme in lipogenesis. Our results show that inhibition of FASN decreased cell viability by ~30%, underscoring the importance of lipogenesis for the survival of melanoma cells. HPLC-MS analyses of the cells showed that the relative abundance of phosphatidylcholine, phosphatidylethanolamine, and phosphatidylinositol PL classes increased, while phosphatidylglycerol (PG), phosphatidylserine, and sphingomyelin (SM) decreased. Furthermore, we determined variations in the composition of the individual molecular species within the PL classes as a function of FASN inhibition. This analysis led to the identification of specific PL molecular species such as 34:1-PG and 40:1-SM, which exhibited more than a ten percent change in their relative abundance upon FASN inhibition, suggesting potentially significant roles in lipogenesis and the survival of melanoma cells (individual PL molecular species are designated as the total number of carbon atoms in the FA chains (or FA chain and sphingosine):total number of double bonds in the FA chains (or FA chain and sphingosine)-name of the PL class). The results from this study offer initial insights into the function of PLs in melanoma pathogenesis.
