Fluorescence Analysis of Carboxyphenyl Porphyrins
Abstract
Studies to analyze the effects of metals on the fluorescence properties of porphyrins were carried out by comparing a metalloporphyrin (Cu(II) meso-Tetra(4-carboxyphenyl) porphyrin, abbreviated as Cu–4- CPP) with it freebase counterpart (meso-Tetra(4-carboxyphenyl) porphyrin, abbreviated as 4-CPP) at various emissions. These porphyrins were selected based on their solubility, structure, and electrochemical properties. A trial was carried out in the UV spectrophotometer to obtain the peak wavelengths of major absorption which were then used to carry out the trials in the spectrofluorometer. In each experiment, the resulting fluorescence peaks were obtained at various emission wavelengths. After analyzing the peaks, it was determined that the metal significantly increases peak intensity at particular emission wavelengths. This research can shed light on the different roles porphyrins and metalloporphyrins have on biochemical reactions that regulate the activity of cardiovascular, neurological, reproductive, and detoxification systems. A computational analysis was performed to correlate the experimental results with the molecular orbital maps.
Faculty Sponsors
Dr. Maria Ballester, Dr. Victor H. Castro Junca, Dr. Brian Van Hoozen
Project Type
Event
Location
Alvin Sherman Library
Start Date
4-3-2024 12:30 PM
End Date
4-4-2024 1:30 PM
Fluorescence Analysis of Carboxyphenyl Porphyrins
Alvin Sherman Library
Studies to analyze the effects of metals on the fluorescence properties of porphyrins were carried out by comparing a metalloporphyrin (Cu(II) meso-Tetra(4-carboxyphenyl) porphyrin, abbreviated as Cu–4- CPP) with it freebase counterpart (meso-Tetra(4-carboxyphenyl) porphyrin, abbreviated as 4-CPP) at various emissions. These porphyrins were selected based on their solubility, structure, and electrochemical properties. A trial was carried out in the UV spectrophotometer to obtain the peak wavelengths of major absorption which were then used to carry out the trials in the spectrofluorometer. In each experiment, the resulting fluorescence peaks were obtained at various emission wavelengths. After analyzing the peaks, it was determined that the metal significantly increases peak intensity at particular emission wavelengths. This research can shed light on the different roles porphyrins and metalloporphyrins have on biochemical reactions that regulate the activity of cardiovascular, neurological, reproductive, and detoxification systems. A computational analysis was performed to correlate the experimental results with the molecular orbital maps.
