A1 vs A2 Milk Variants and their Impact on Cysteine Metabolism in Autism-Spectrum Disorder
Abstract
Dairy cow milk stands as a vital source of nutrients encompassing proteins, carbohydrates, fats, and micronutrients. This nutritional complexity is heightened by the choice between A1 and A2 milk types. This research probes the distinctions between A1 and A2 milk and their potential implications for cysteine metabolism within the context of autism spectrum disorder (ASD). This study focuses on BCM7, a bioactive opioid peptide exclusively released during digestion of A1 milk, and its impact on mitochondrial respiration of macrophage-like THP1 cells.
Using the Seahorse XF for mitochondrial assessment, oxygen consumption rate (OCR), extracellular acidification rate (ECAR), basal respiration, proton leak, maximal respiration, spare respiratory capacity, non-mitochondrial oxygen consumption, ATP production coupled respiration, and coupling efficiency were measured for PMA-treated THP1 cells as well as BCM7 treated THP1 cells.
Basal respiration increased in experimental cells by 8.52 pmol/min and ATP production coupled respiration by 4.35 pmol/min. However, the results revealed no significant change (p < 0.05) of BCM7 on THP1 cells. For example, mitochondrial respiration, non-mitochondrial oxygen consumption, and coupling efficiency showed only minimal changes. Maximal respiration and spare respiratory capacity decreased in the BCM7 treated cells by 23.4 pmol/min and 31.9 pmol/min respectively. In conclusion, the findings of this study indicate that BCM7 does not exert a significant influence on mitochondrial respiration in THP-1 cells under the conditions tested.
Faculty Sponsors
Dr. Richard Deth, Dr. Benedict Albensi
Project Type
Event
Location
Alvin Sherman Library
Start Date
4-3-2024 12:30 PM
End Date
4-4-2024 1:30 PM
A1 vs A2 Milk Variants and their Impact on Cysteine Metabolism in Autism-Spectrum Disorder
Alvin Sherman Library
Dairy cow milk stands as a vital source of nutrients encompassing proteins, carbohydrates, fats, and micronutrients. This nutritional complexity is heightened by the choice between A1 and A2 milk types. This research probes the distinctions between A1 and A2 milk and their potential implications for cysteine metabolism within the context of autism spectrum disorder (ASD). This study focuses on BCM7, a bioactive opioid peptide exclusively released during digestion of A1 milk, and its impact on mitochondrial respiration of macrophage-like THP1 cells.
Using the Seahorse XF for mitochondrial assessment, oxygen consumption rate (OCR), extracellular acidification rate (ECAR), basal respiration, proton leak, maximal respiration, spare respiratory capacity, non-mitochondrial oxygen consumption, ATP production coupled respiration, and coupling efficiency were measured for PMA-treated THP1 cells as well as BCM7 treated THP1 cells.
Basal respiration increased in experimental cells by 8.52 pmol/min and ATP production coupled respiration by 4.35 pmol/min. However, the results revealed no significant change (p < 0.05) of BCM7 on THP1 cells. For example, mitochondrial respiration, non-mitochondrial oxygen consumption, and coupling efficiency showed only minimal changes. Maximal respiration and spare respiratory capacity decreased in the BCM7 treated cells by 23.4 pmol/min and 31.9 pmol/min respectively. In conclusion, the findings of this study indicate that BCM7 does not exert a significant influence on mitochondrial respiration in THP-1 cells under the conditions tested.
