S-PRG Eluate Downregulates Osteoclastogenesis: S-PRG's Possible Indication for Subgingival Caries
Abstract
Surface pre-reacted glass-ionomer (S-PRG) is a new bioactive filler that has been used for the restoration of tooth decay. S-PRG’s unique property to release six types of bioactive ions, including fluoride (F−), prevents the adhesion of dental plaque to tooth surface while suppressing tooth demineralization. Although S-PRG may be potentially used for subgingival caries restoration, the possible effect of S-PRG on the pathogenically promoted bone absorption in periodontitis remains unclear. The aim of this study is to investigate the possible inhibitory effect of S-PRG eluate on osteoclasto-genesis (OC-genesis) in vitro. S-PRG elute at the sublethal concentrations, which were formerly determined by WST8-viability assay, were applied to RAW264.7 cells stimulated with or without OC-genesis factor RANKL (50 ng/ml). TRAP staining and pit formation assays were conducted to monitor the effects of S-PRG eluate on the OC-genesis and OCs’ bone-resorption function, respectively. S-PRG eluate’s effect on the expressions of genes associated with OC-genesis, including, ocstamp, dcstamp, cathepsinK, nfatc1, and acp5, was measured using Q-PCR. NFATc1, a pivotal OCgenesis transcription factor, was measured using W-blot. At the concentrations tested, S-PRG significantly suppressed OC-genesis from RANKL-primed RAW264.7 cells. The expressions of all OCgenesis-related genes induced by RANKL were significantly down-regulated by S-PRG eluate in a dosedependent manner. RANKL-mediated induction of NFATc1 was suppressed by S-PRG eluate, suggesting that S-PRG inhibits the RANKL-elicited OC-genesis via suppression of transcription factor required for OC-genesis. These results suggest the possible indication of S-PRG for subgingival caries restoration, due to its effect to prevent periodontitis.
Faculty Sponsors
Dr. Shin Nakamura, Dr. Toshihisa Kawai
Project Type
Event
Location
Alvin Sherman Library
Start Date
4-5-2023 12:00 PM
End Date
4-6-2023 4:00 PM
S-PRG Eluate Downregulates Osteoclastogenesis: S-PRG's Possible Indication for Subgingival Caries
Alvin Sherman Library
Surface pre-reacted glass-ionomer (S-PRG) is a new bioactive filler that has been used for the restoration of tooth decay. S-PRG’s unique property to release six types of bioactive ions, including fluoride (F−), prevents the adhesion of dental plaque to tooth surface while suppressing tooth demineralization. Although S-PRG may be potentially used for subgingival caries restoration, the possible effect of S-PRG on the pathogenically promoted bone absorption in periodontitis remains unclear. The aim of this study is to investigate the possible inhibitory effect of S-PRG eluate on osteoclasto-genesis (OC-genesis) in vitro. S-PRG elute at the sublethal concentrations, which were formerly determined by WST8-viability assay, were applied to RAW264.7 cells stimulated with or without OC-genesis factor RANKL (50 ng/ml). TRAP staining and pit formation assays were conducted to monitor the effects of S-PRG eluate on the OC-genesis and OCs’ bone-resorption function, respectively. S-PRG eluate’s effect on the expressions of genes associated with OC-genesis, including, ocstamp, dcstamp, cathepsinK, nfatc1, and acp5, was measured using Q-PCR. NFATc1, a pivotal OCgenesis transcription factor, was measured using W-blot. At the concentrations tested, S-PRG significantly suppressed OC-genesis from RANKL-primed RAW264.7 cells. The expressions of all OCgenesis-related genes induced by RANKL were significantly down-regulated by S-PRG eluate in a dosedependent manner. RANKL-mediated induction of NFATc1 was suppressed by S-PRG eluate, suggesting that S-PRG inhibits the RANKL-elicited OC-genesis via suppression of transcription factor required for OC-genesis. These results suggest the possible indication of S-PRG for subgingival caries restoration, due to its effect to prevent periodontitis.
