Effect of Oral Microbiome Components on SARS-CoV-2 Infection
Abstract
Using an in vitro infection model of SARS-CoV-2 infection utilizing pseudotype lentivirus, we aim to study the effects of the oral microbiome in COVID-19 infection. The model is based on the production of pseudotype lentivirus targeting 293 cell lines expressing the viral receptor Ace 2. Looking at extrinsic factors, it has been observed that bacteria exert an effect on viral infection. Common bacteria species found composing the oral microbiome include S. mitis, C. Gingivalis, A.a. 29523, A.a. Y4, S. gordoni, S. gordoni, Pi, and A. Odontitis were screened. Preliminary data shows that the oral microbiota has the potential to facilitate or inhibit SARS-CoV-2 infection. The experiments include preparing plates with 293T-Ace 2+ cells, pretreating the cells in triplicates, and pretreating the virus with the same factors prior to infection. These experiments will shed light on the extent to which the oral microbiome affects SARS-CoV-2 infection as well as identify groups that have enhancing, inhibiting, or no effect on infection. Considering the bacterial factors analyzed in these experiments are common to the oral microbiome they have the potential to serve as therapeutics for a preventative treatment against SARSCoV-2 infection. The high rates of transmission and high mortality rate of SARS-CoV-2 make this project crucial. Identifying therapeutics for a preventative treatment that is native to the human body can aid in providing a preventative measure to immunocompromised populations, as well as those with vaccine hesitancy.
Faculty Sponsors
Dr. Mark Cayabyab, Dr. Alexander Bontempo
Project Type
Event
Location
Alvin Sherman Library
Start Date
4-5-2023 12:00 PM
End Date
4-6-2023 4:00 PM
Effect of Oral Microbiome Components on SARS-CoV-2 Infection
Alvin Sherman Library
Using an in vitro infection model of SARS-CoV-2 infection utilizing pseudotype lentivirus, we aim to study the effects of the oral microbiome in COVID-19 infection. The model is based on the production of pseudotype lentivirus targeting 293 cell lines expressing the viral receptor Ace 2. Looking at extrinsic factors, it has been observed that bacteria exert an effect on viral infection. Common bacteria species found composing the oral microbiome include S. mitis, C. Gingivalis, A.a. 29523, A.a. Y4, S. gordoni, S. gordoni, Pi, and A. Odontitis were screened. Preliminary data shows that the oral microbiota has the potential to facilitate or inhibit SARS-CoV-2 infection. The experiments include preparing plates with 293T-Ace 2+ cells, pretreating the cells in triplicates, and pretreating the virus with the same factors prior to infection. These experiments will shed light on the extent to which the oral microbiome affects SARS-CoV-2 infection as well as identify groups that have enhancing, inhibiting, or no effect on infection. Considering the bacterial factors analyzed in these experiments are common to the oral microbiome they have the potential to serve as therapeutics for a preventative treatment against SARSCoV-2 infection. The high rates of transmission and high mortality rate of SARS-CoV-2 make this project crucial. Identifying therapeutics for a preventative treatment that is native to the human body can aid in providing a preventative measure to immunocompromised populations, as well as those with vaccine hesitancy.
