VEGF Induced Endothelial Marker Gene Expression in Periodontal Ligament Derived Stem Cells (PDLSCs)
Abstract
Background: Establishing endothelial-cell mediated vascularization is crucial in bone tissue engineering, as blood vessels are key regulators for newly formed bone homeostasis and growth. Periodontal ligament stem cells (PDLSCs), derived from periodontal ligament tissue can differentiate into a wide range of cell types, including hepatocytes, osteoblasts, chondrocytes, and adipocytes. However, there is not much research focused on their ability to differentiate into endothelial cells. Objective: The objective of this study was to investigate the ability of PDSCs differentiate into endothelial cells. Methodology: Human PDLSCs were harvested from impacted wisdom teeth from NSU clinics. The cells were cultured using standard protocols. Third or fourth passage cultures were used for all experiments. To induce endothelial differentiation, PDLSCs grown in complete medium (DMEM, 10% FBS, and 1% antibiotics) were treated with varied concentrations of VEGF, (10, 50, and 100 ng/ml). PDLSCs grown in complete medium alone were designated as the control. The expression of endothelial marker genes, vascular cell adhesion molecule (VCAM-1), kinase insert domain-containing receptor (KDR), and FMS related tyrosine kinase-1 (FLT-1) were measured using quantitative PCR. Results: Our results demonstrated the up regulation of all endothelial markers gene expression compared to cells grown in the absence of VEGF. Conclusion: PDLSCs have potential to differentiate into endothelial cells, thus supporting the formation of vascularized bone.
Faculty Sponsors
Dr. Umadevi Kandalam
Project Type
Event
Location
Alvin Shermany Library
Start Date
4-5-2019 1:00 PM
End Date
4-5-2019 5:00 PM
VEGF Induced Endothelial Marker Gene Expression in Periodontal Ligament Derived Stem Cells (PDLSCs)
Alvin Shermany Library
Background: Establishing endothelial-cell mediated vascularization is crucial in bone tissue engineering, as blood vessels are key regulators for newly formed bone homeostasis and growth. Periodontal ligament stem cells (PDLSCs), derived from periodontal ligament tissue can differentiate into a wide range of cell types, including hepatocytes, osteoblasts, chondrocytes, and adipocytes. However, there is not much research focused on their ability to differentiate into endothelial cells. Objective: The objective of this study was to investigate the ability of PDSCs differentiate into endothelial cells. Methodology: Human PDLSCs were harvested from impacted wisdom teeth from NSU clinics. The cells were cultured using standard protocols. Third or fourth passage cultures were used for all experiments. To induce endothelial differentiation, PDLSCs grown in complete medium (DMEM, 10% FBS, and 1% antibiotics) were treated with varied concentrations of VEGF, (10, 50, and 100 ng/ml). PDLSCs grown in complete medium alone were designated as the control. The expression of endothelial marker genes, vascular cell adhesion molecule (VCAM-1), kinase insert domain-containing receptor (KDR), and FMS related tyrosine kinase-1 (FLT-1) were measured using quantitative PCR. Results: Our results demonstrated the up regulation of all endothelial markers gene expression compared to cells grown in the absence of VEGF. Conclusion: PDLSCs have potential to differentiate into endothelial cells, thus supporting the formation of vascularized bone.
