Release Kinetics of Anti-Sema4D Monoclonal Antibody from Alginate Beads
Abstract
Background/Introduction: The reconstruction of bone in critical size defects presents clinical challenges in cranio and oral maxillofacial surgery. The long-term goal of this project is to regenerate a resilient bone for the repair of these defects. A recent study revaled that Semaphorin 4D (Sema4D), a pleiotropic transmembrane glycoprotein, expressed by osteoclasts inhibits osteoblast differentiation. Our hypothesis holds that local delivery of anti-Sema4D monoclonal antibody (mAb) adjunct to bone-regenerative cell-therapy may enhance osteoblast differentiation. However, currently, no methodology is available for the local mAb delivery. In this study, we intended to develop a novel alginate-based anti-Sema4D-mAb delivery system. Objective: The objective of the study is to examine the release kinetics of anti-Sema4D-mAb from alginate beads. Materials/Methods: Alginate beads (2% alginate) containing various concentrations (0, 5 and 10 μg) of anti-Sema4D-mAb, generated by Dr. Kawai's group (College of Dental Medicine, NSU) were prepared. Anti-Sema4D-mAb embedded beads in PBS were incubated in a 6-well culture-plate at 37°C. The release of anti-Sema4D mAb after incubation for various periods (1-120 hrs) was measured by ELISA using a Sema4D-peptide as target antigen. Results: There was a steady increase of anti-Sema4D-mAb release from alginate beads which peaked at 24 hours, followed by a gradual decrease in the release at 30-120 hours which still maintained at least 30% level compared to its peak. Furthermore, positive reaction in Sema4D peptide ELISA indicated that anti-Sema4D-mAb's binding function was retained in alginate beads. Conclusion: Alginate appeared to be an excellent biomaterial for the extended release of anti-Sema4D-mAb.
Faculty Sponsors
Dr. Umadevi Kandalam
Project Type
Event
Location
Alvin Shermany Library
Start Date
4-5-2019 1:00 PM
End Date
4-5-2019 5:00 PM
Release Kinetics of Anti-Sema4D Monoclonal Antibody from Alginate Beads
Alvin Shermany Library
Background/Introduction: The reconstruction of bone in critical size defects presents clinical challenges in cranio and oral maxillofacial surgery. The long-term goal of this project is to regenerate a resilient bone for the repair of these defects. A recent study revaled that Semaphorin 4D (Sema4D), a pleiotropic transmembrane glycoprotein, expressed by osteoclasts inhibits osteoblast differentiation. Our hypothesis holds that local delivery of anti-Sema4D monoclonal antibody (mAb) adjunct to bone-regenerative cell-therapy may enhance osteoblast differentiation. However, currently, no methodology is available for the local mAb delivery. In this study, we intended to develop a novel alginate-based anti-Sema4D-mAb delivery system. Objective: The objective of the study is to examine the release kinetics of anti-Sema4D-mAb from alginate beads. Materials/Methods: Alginate beads (2% alginate) containing various concentrations (0, 5 and 10 μg) of anti-Sema4D-mAb, generated by Dr. Kawai's group (College of Dental Medicine, NSU) were prepared. Anti-Sema4D-mAb embedded beads in PBS were incubated in a 6-well culture-plate at 37°C. The release of anti-Sema4D mAb after incubation for various periods (1-120 hrs) was measured by ELISA using a Sema4D-peptide as target antigen. Results: There was a steady increase of anti-Sema4D-mAb release from alginate beads which peaked at 24 hours, followed by a gradual decrease in the release at 30-120 hours which still maintained at least 30% level compared to its peak. Furthermore, positive reaction in Sema4D peptide ELISA indicated that anti-Sema4D-mAb's binding function was retained in alginate beads. Conclusion: Alginate appeared to be an excellent biomaterial for the extended release of anti-Sema4D-mAb.
