Examining the Antibacterial Properties of Placental Tissue Components
Abstract
As the use of antibiotics has increased, the rate of antibiotic resistance has grown leading to a pressure to develop new antibiotics. Placental tissue is known to exhibit antimicrobial activity against bacteria, making it a viable candidate for novel antibiotic development. With this knowledge in hand, various components of placental tissue were tested to determine the portions that exhibited antibacterial activity. Dried placental tissue were micronized and rehydrated. The goal of this project was to take the product and test them on multiple bacterial species, some of which are common to human skin. The bacterial species tested were Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Mycobacterium smegmatis, and MRSA. The bacteria were inoculated onto nutrient broth, incubated overnight at 37°C, and then diluted in water to match the turbidity of a McFarland Standard. The bacteria were spread on Mueller-Hinton Agar plates and a disk diffusion method was used. Disks with 20 uL of the hydrated tissue were placed on the inoculated plates and incubated at 37°C overnight. Analysis of the plates showed that many of the placental tissues had a zone of inhibition around the disks, which indicated antibacterial activity. To determine whether the components exhibited bactericidal or bacteriostatic properties, bacteria within the zones of inhibition were collected, streaked onto nutrient agar plates and incubated overnight at 37°C. The analysis showed the tissue components had a bacteriostatic mode of inhibition, which indicates its potential in applications intended to promote healing.
Faculty Sponsors
Dr. Julie Torruellas Garcia, Dr. Wendy Weston
Project Type
Event
Location
Alvin Shermany Library
Start Date
4-5-2019 1:00 PM
End Date
4-5-2019 5:00 PM
Examining the Antibacterial Properties of Placental Tissue Components
Alvin Shermany Library
As the use of antibiotics has increased, the rate of antibiotic resistance has grown leading to a pressure to develop new antibiotics. Placental tissue is known to exhibit antimicrobial activity against bacteria, making it a viable candidate for novel antibiotic development. With this knowledge in hand, various components of placental tissue were tested to determine the portions that exhibited antibacterial activity. Dried placental tissue were micronized and rehydrated. The goal of this project was to take the product and test them on multiple bacterial species, some of which are common to human skin. The bacterial species tested were Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Mycobacterium smegmatis, and MRSA. The bacteria were inoculated onto nutrient broth, incubated overnight at 37°C, and then diluted in water to match the turbidity of a McFarland Standard. The bacteria were spread on Mueller-Hinton Agar plates and a disk diffusion method was used. Disks with 20 uL of the hydrated tissue were placed on the inoculated plates and incubated at 37°C overnight. Analysis of the plates showed that many of the placental tissues had a zone of inhibition around the disks, which indicated antibacterial activity. To determine whether the components exhibited bactericidal or bacteriostatic properties, bacteria within the zones of inhibition were collected, streaked onto nutrient agar plates and incubated overnight at 37°C. The analysis showed the tissue components had a bacteriostatic mode of inhibition, which indicates its potential in applications intended to promote healing.
