Evaluating the Efficiency of the Clorox® Total 360®System to Eliminate Bacteria on Hard-to- Reach Surfaces

Evaluating the Efficiency of the Clorox® Total 360®System to Eliminate Bacteria on Hard-to- Reach Surfaces

Electrostatic Sprayers have gained popularity in a wide range of industrial settings, ranging from pest to bacterial control for both agricultural and commercial use. The Clorox® Total 360®System uses an industrial electrostatic sprayer that adds a charge to the liquid particles of the Clorox® Total 360® Disinfectant and thus enhances the disinfectant’s ability to adhere to surfaces. The goal of this investigation was to test the efficacy of the Clorox® Total 360®System in delivering Clorox® Total 360® Disinfectant Cleaner to hard-to-reach surfaces and to determine if the ionized disinfectant particles can wrap around objects to kill bacteria on the front and back. A coverslip containing 106 E. coli was affixed to either the front or the back of a 250mL graduated cylinder. Disinfectant was sprayed for one or three seconds from three feet away. Controls were sprayed with water only. After 3 minutes, the bacteria were diluted, filtered and plated on nutrient agar. After overnight incubation at 37°C, the colonies were counted, and the percent and log reductions were calculated. The experiments were repeated using a traditional spray bottle for comparison. The Clorox® Total 360®System resulted in 99.9% reduction of E. coli compared to 82.6% for the spray bottle. When analyzing the ability to wrap around an object, the Clorox® Total 360®System resulted in 58.6% reduction of bacteria compared to 22.6% for the spray bottle. In conclusion, the Clorox® Total 360®System was more effective at wrapping the disinfectant around objects and killing bacteria when compared to a traditional spray bottle.