Abstract
Background: Cleft palate is the second most common congenital defect in the US. This genetic disability can be extremely debilitating affecting individual’s speech and facial growth. Bone tissue engineering emerged as a potential option for the repair and regeneration of the bone in the defect area. Objective: The objective of the study was to investigate proliferation and osteogenic differentiation of periodontal ligament derived stem cells (PDLSCs) encapsulated in a self-assembling, nano-fiber hydrogel scaffold, Puramatrix™. Methodology: PDLSCs were cultured in Puramatrix™. The cell proliferation was measured by a colorimetric WST assay at 1, 2, 3 and 5 day intervals. Cell viability was assessed using live dead cell assay. PDLSCs encapsulated in Puramatrix™ were either grown in the complete medium (CM), which served as the control, or osteogenic medium (OM). The expression of osteogenic marker genes alkaline phosphatase (ALP) and collagen type I (COLI) were assessed by quantitative PCR. The results were analyzed by one-way ANOVA. Results: Our results revealed that PDLSCs were viable at all concentrations observed. There was significant increase (P<0.05) in the proliferation in the cells seeded with (0.1 and 3) x105 /ml from day 1 to Day 5. The viability of the cells seeded with (30x105 )/ml were comparable with control. The results of live dead cell assay demonstrated PDLSCs encapsulated in Puramatrix™ survived at all concentrations. There was a twofold upregulation of ALP, COL I in Puramatrix™ encapsulated cells compared to control. Conclusion: Puramatrix™ supported PDLSCs growth and differentiation. This tissue engineered construct has potential applications for bone regeneration.
Faculty Sponsors
Dr. Umadevi Kandalam
Project Type
Event
Location
Alvin Sherman Library
Start Date
4-7-2017 12:00 AM
End Date
4-7-2017 12:00 AM
Proliferation and Osteogenic Differentiation of Dental Stem Cells in a Nanofibrous Hydrogel
Alvin Sherman Library
Background: Cleft palate is the second most common congenital defect in the US. This genetic disability can be extremely debilitating affecting individual’s speech and facial growth. Bone tissue engineering emerged as a potential option for the repair and regeneration of the bone in the defect area. Objective: The objective of the study was to investigate proliferation and osteogenic differentiation of periodontal ligament derived stem cells (PDLSCs) encapsulated in a self-assembling, nano-fiber hydrogel scaffold, Puramatrix™. Methodology: PDLSCs were cultured in Puramatrix™. The cell proliferation was measured by a colorimetric WST assay at 1, 2, 3 and 5 day intervals. Cell viability was assessed using live dead cell assay. PDLSCs encapsulated in Puramatrix™ were either grown in the complete medium (CM), which served as the control, or osteogenic medium (OM). The expression of osteogenic marker genes alkaline phosphatase (ALP) and collagen type I (COLI) were assessed by quantitative PCR. The results were analyzed by one-way ANOVA. Results: Our results revealed that PDLSCs were viable at all concentrations observed. There was significant increase (P<0.05) in the proliferation in the cells seeded with (0.1 and 3) x105 /ml from day 1 to Day 5. The viability of the cells seeded with (30x105 )/ml were comparable with control. The results of live dead cell assay demonstrated PDLSCs encapsulated in Puramatrix™ survived at all concentrations. There was a twofold upregulation of ALP, COL I in Puramatrix™ encapsulated cells compared to control. Conclusion: Puramatrix™ supported PDLSCs growth and differentiation. This tissue engineered construct has potential applications for bone regeneration.
