Abstract
The purpose of this research was to identify and quantify lipids in the eastern oyster, Crassostrea virginica. Gas chromatography was used to separate the lipids found in the oyster samples, while mass spectrometry was used for analysis. Retention times of the sample were compared to known lipid standard retention times in order to identify the lipids. The lipids were quantified utilizing a lipid standard that is not present in the oyster. Using this same technique, oysters from various locations can be analyzed to see if there are any differences in the types of lipids or the quantity of lipid. It is hypothesized that oysters under stress may have lower lipid content. By comparing oysters from outside the Gulf of Mexico to oysters from that region, during and after the BP oil spill, it can be determined whether or not this event had an effect on the lipids of the eastern oyster.
Faculty Sponsors
Dr. Deanne Roopnarine
Project Type
Event
Location
Alvin Sherman Library
Start Date
4-8-2016 1:00 PM
End Date
4-8-2016 5:30 PM
Identification and Quantification of Lipid Content in Oysters Using GC Mass Spectrometry
Alvin Sherman Library
The purpose of this research was to identify and quantify lipids in the eastern oyster, Crassostrea virginica. Gas chromatography was used to separate the lipids found in the oyster samples, while mass spectrometry was used for analysis. Retention times of the sample were compared to known lipid standard retention times in order to identify the lipids. The lipids were quantified utilizing a lipid standard that is not present in the oyster. Using this same technique, oysters from various locations can be analyzed to see if there are any differences in the types of lipids or the quantity of lipid. It is hypothesized that oysters under stress may have lower lipid content. By comparing oysters from outside the Gulf of Mexico to oysters from that region, during and after the BP oil spill, it can be determined whether or not this event had an effect on the lipids of the eastern oyster.
