Abstract

Continuing research and development has led to new advancements in sanitation technology which allows for better preventative techniques against disease and illnesses. A total solutions company, ByoPlanet, developed an electrostatic sprayer that applies a negative charge to a disinfectant. This permits a stronger attraction to the environment as objects are naturally positively charged. Our goal was to determine if the electrostatic sprayer was able to treat objects that are not in the direct line of contact with the spray. The treatment was Clorox Hydrogen Peroxide and water was used as a control. The electrostatic sprayer was placed three feet from a cylindrical beaker that had two cover slips attached in direct opposite sides from each other. Each cover slip contained either 106 Escherichia coli. In each trial, the electrostatic sprayer was sprayed for three seconds. The disinfectant was allowed to interact with the bacteria for three minutes. The cover slips were removed from the beaker, diluted and plated on Nutrient Agar, and incubated overnight at 37°C. Next, colonies were counted to determine the percent reduction and the log reduction of bacteria. As a control, the same procedure was performed with a traditional spray bottle, except it was sprayed five times in three seconds. A comparison of the percent reduction and log reduction between both cover slips was done to determine if the electrostatic sprayer could deliver disinfectant to areas not in the direct line of contact of the spray.

Faculty Sponsors

Dr. Julie Torruellas Garcia

Project Type

Event

Location

Alvin Sherman Library

Start Date

4-8-2016 1:00 PM

End Date

4-8-2016 5:30 PM

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Apr 8th, 1:00 PM Apr 8th, 5:30 PM

Efficiency of an Electrostatic Sprayer in the Application of Disinfectant

Alvin Sherman Library

Continuing research and development has led to new advancements in sanitation technology which allows for better preventative techniques against disease and illnesses. A total solutions company, ByoPlanet, developed an electrostatic sprayer that applies a negative charge to a disinfectant. This permits a stronger attraction to the environment as objects are naturally positively charged. Our goal was to determine if the electrostatic sprayer was able to treat objects that are not in the direct line of contact with the spray. The treatment was Clorox Hydrogen Peroxide and water was used as a control. The electrostatic sprayer was placed three feet from a cylindrical beaker that had two cover slips attached in direct opposite sides from each other. Each cover slip contained either 106 Escherichia coli. In each trial, the electrostatic sprayer was sprayed for three seconds. The disinfectant was allowed to interact with the bacteria for three minutes. The cover slips were removed from the beaker, diluted and plated on Nutrient Agar, and incubated overnight at 37°C. Next, colonies were counted to determine the percent reduction and the log reduction of bacteria. As a control, the same procedure was performed with a traditional spray bottle, except it was sprayed five times in three seconds. A comparison of the percent reduction and log reduction between both cover slips was done to determine if the electrostatic sprayer could deliver disinfectant to areas not in the direct line of contact of the spray.