Abstract
Objective: The aim of this study was to investigate the effect of bromelain on osteogenic differentiation of human gingiva derived mesenchymal stem cells (HGMSCs). Background: HGMSCs are highly proliferative with the ability to differentiate into osteogenic precursor cells. While dexamethasone is a traditional inducer of osteogenic differentiation, many antioxidants play a vital role in enhancing osteogenic differentiation and offer a potential alternative to dexamethasone. Bromelain, an antioxidant derived from pineapple extract is an antioxidant and anti-inflammatory agent. Studies have shown that bromelain inhibits adipogenic differentiation. Methodology: Cryopreserved HGMSCs were used for the study. The cells obtained from passage 4 were used for the study. The cells were treated with various concentrations (1, 2.5, and 5 μg/ml) of bromelain. Cells treated with dexamethasone was used as control. Two weeks after post induction, osteopontin (which is a osteogenic marker protein) expression was investigated. Results: The western blotting results demonstrated 50% upregulation in osteopontin protein expression in that the cells induced with bromelain at 1, 2.5 μg/ml for 2 weeks when compared with cells treated with dexamethasone. Conclusion: The findings from the study demonstrated that bromelain treatment induced osteogenic differentiation of HGMSCs. Funding: HPD grant
Faculty Sponsors
Dr. Umadevi Kandalam
Project Type
Event
Location
Alvin Sherman Library
Start Date
4-8-2016 1:00 PM
End Date
4-8-2016 5:30 PM
Bromelain Induces Osteogenic Differentiation in Human Gingiva Derived Stem Cells
Alvin Sherman Library
Objective: The aim of this study was to investigate the effect of bromelain on osteogenic differentiation of human gingiva derived mesenchymal stem cells (HGMSCs). Background: HGMSCs are highly proliferative with the ability to differentiate into osteogenic precursor cells. While dexamethasone is a traditional inducer of osteogenic differentiation, many antioxidants play a vital role in enhancing osteogenic differentiation and offer a potential alternative to dexamethasone. Bromelain, an antioxidant derived from pineapple extract is an antioxidant and anti-inflammatory agent. Studies have shown that bromelain inhibits adipogenic differentiation. Methodology: Cryopreserved HGMSCs were used for the study. The cells obtained from passage 4 were used for the study. The cells were treated with various concentrations (1, 2.5, and 5 μg/ml) of bromelain. Cells treated with dexamethasone was used as control. Two weeks after post induction, osteopontin (which is a osteogenic marker protein) expression was investigated. Results: The western blotting results demonstrated 50% upregulation in osteopontin protein expression in that the cells induced with bromelain at 1, 2.5 μg/ml for 2 weeks when compared with cells treated with dexamethasone. Conclusion: The findings from the study demonstrated that bromelain treatment induced osteogenic differentiation of HGMSCs. Funding: HPD grant
