Abstract
In today’s effort to improve public health, antimicrobials have been a key point of focus in reducing the spread of infectious disease. ByoPlanet, a total solutions provider for bringing advanced science for killing today’s germs, took on the challenge of developing new products for sanitation in its Advanced Oxidation Prototype. In this system, distilled water is exposed to UV light and infused with ozone. Our goal was to determine if the treated water exhibited antibacterial activity. Ten liters of distilled water was pumped through the Advanced Oxidation Prototype for 15 minutes. The treated water was applied to Escherichia coli or Staphylococcus aureus for ten minutes and serial dilution plating onto nutrient agar was used to determine the percent reduction of the bacteria. Distilled water was used as a control. Plates were incubated overnight at 37°C and colonies were counted post incubation. The percent reduction was calculated by subtracting the number of bacteria on the test plates from the control plates, dividing by the number of bacteria on the control plates and multiplying by 100. All experiments were performed in triplicate. The results showed that the treated water led to an 87% reduction of growth of E. coli and a 68% reduction of growth of S. aureus. The goal is to have a 99.999% reduction as seen in ByoPlanet’s Vital Oxide Disinfectant. This experiment shows that with some modifications, the Advanced Oxidation Prototype has potential use as a novel disinfectant. This project was funded by ByoPlanet International.
Faculty Sponsors
Julie Torruellas Garcia, Ph.D.
Project Type
Event
Location
Alvin Sherman Library
Start Date
4-10-2015 1:00 PM
End Date
4-10-2015 5:30 PM
Antibacterial Activity of ByoPlanet's Advanced Oxidation Prototype
Alvin Sherman Library
In today’s effort to improve public health, antimicrobials have been a key point of focus in reducing the spread of infectious disease. ByoPlanet, a total solutions provider for bringing advanced science for killing today’s germs, took on the challenge of developing new products for sanitation in its Advanced Oxidation Prototype. In this system, distilled water is exposed to UV light and infused with ozone. Our goal was to determine if the treated water exhibited antibacterial activity. Ten liters of distilled water was pumped through the Advanced Oxidation Prototype for 15 minutes. The treated water was applied to Escherichia coli or Staphylococcus aureus for ten minutes and serial dilution plating onto nutrient agar was used to determine the percent reduction of the bacteria. Distilled water was used as a control. Plates were incubated overnight at 37°C and colonies were counted post incubation. The percent reduction was calculated by subtracting the number of bacteria on the test plates from the control plates, dividing by the number of bacteria on the control plates and multiplying by 100. All experiments were performed in triplicate. The results showed that the treated water led to an 87% reduction of growth of E. coli and a 68% reduction of growth of S. aureus. The goal is to have a 99.999% reduction as seen in ByoPlanet’s Vital Oxide Disinfectant. This experiment shows that with some modifications, the Advanced Oxidation Prototype has potential use as a novel disinfectant. This project was funded by ByoPlanet International.
