Abstract
Introduction: Bone Regeneration in craniofacial region due to traumatic injuries and congenital defects is a major clinical problem. Cleft palate is the second most common congenital defect. Our long-term goal is to regenerate bone in the palatal region using tissue-engineering approaches. Bone tissue engineering utilizes osteogenic cells, osteoconductive scaffolds and osteoinductive signals. Mesenchymal stem cells derived from human umbilical cord (HUMSCs) are highly proliferative with the ability to differentiate into osteogenic precursor cells. Objective: The primary objective of the study was to encapsulate HUMSCs in PuraMatrix™ (PM), a peptide hydrogel, and investigate their survival and osteogenic differentiation. PM is an injectable 3D nanofiber hydrogel scaffold capable of self-assembly when exposed to physiologic conditions. Methodology: HUMSCs as monolayer cultures were plated for 3-4 weeks in either complete medium (CM) or osteogenic medium (OM). Gene expression, protein expression, and alkaline phosphatase markers were used to analyze osteogenic differentiation. To determine the cytocompatibility and osteogenic differentiation HUMSCs and cells were encapsulated in Puramatrix cell viability and osteogenic differentiation was examined. Results: Live-Dead cell assays showed that cells implanted in Puramatrix were viable. A two- fold upregulation of Alkaline phosphatase (ALP) gene was observed in cells encapsulated in PM with OM compared to cells in CM (p<0.05). Conclusion: Puramatrix supports the cell growth and osteogenic potential of HUMSCs.
Faculty Sponsors
Umadevi Kandalam, Ph.D.
Project Type
Event
Location
Alvin Sherman Library
Start Date
4-10-2015 1:00 PM
End Date
4-10-2015 5:30 PM
Osteogenic Differentiation of Human Umbilical Cord Derived Stem Cells in a 3-Dimensional (3-D) Hydrogel
Alvin Sherman Library
Introduction: Bone Regeneration in craniofacial region due to traumatic injuries and congenital defects is a major clinical problem. Cleft palate is the second most common congenital defect. Our long-term goal is to regenerate bone in the palatal region using tissue-engineering approaches. Bone tissue engineering utilizes osteogenic cells, osteoconductive scaffolds and osteoinductive signals. Mesenchymal stem cells derived from human umbilical cord (HUMSCs) are highly proliferative with the ability to differentiate into osteogenic precursor cells. Objective: The primary objective of the study was to encapsulate HUMSCs in PuraMatrix™ (PM), a peptide hydrogel, and investigate their survival and osteogenic differentiation. PM is an injectable 3D nanofiber hydrogel scaffold capable of self-assembly when exposed to physiologic conditions. Methodology: HUMSCs as monolayer cultures were plated for 3-4 weeks in either complete medium (CM) or osteogenic medium (OM). Gene expression, protein expression, and alkaline phosphatase markers were used to analyze osteogenic differentiation. To determine the cytocompatibility and osteogenic differentiation HUMSCs and cells were encapsulated in Puramatrix cell viability and osteogenic differentiation was examined. Results: Live-Dead cell assays showed that cells implanted in Puramatrix were viable. A two- fold upregulation of Alkaline phosphatase (ALP) gene was observed in cells encapsulated in PM with OM compared to cells in CM (p<0.05). Conclusion: Puramatrix supports the cell growth and osteogenic potential of HUMSCs.
